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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: A trans fatty acid substitute enhanced development of liver proliferative lesions induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet

Fig. 4

Pathway analysis and hepatic status of apoptosis and NF-κB signaling at the end of week 13. The genes were selected by expression analysis for comparisons between the control, CDAA-HF-T(+), and CDAA-HF-T(−) groups, with an FDR P value < 0.05 and/or FC > ±1.5. Activated disease or functional annotation (|z-score| ≥ 2) for DEGs in Ingenuity Pathway Analysis (a). Densitometric outcomes of the immunoblot analyses of cleaved caspase 3 and phosphorylated NF-κB-p65 and I-κBα levels expressed as the ratios versus the caspase 3, NF-κB-p65, and GAPDH levels, respectively. The control diet (1), CDAA-HF-T(+) (2), and CDAA-HF-T(−) (3) groups. The following values are presented as the mean + SD. Cleaved caspase 3 / caspase 3: control chow, 1.00 ± 0.05; CDAA-HF-T(+), 1.61 ± 0.26; CDAA-HF-T(−), 2.43 ± 0.61. Phosphorylated NF-κB-p65 / NF-κB-p65: control chow, 1.00 ± 0.17; CDAA-HF-T(+), 0.33 ± 0.17; CDAA-HF-T(−), 0.23 ± 0.14. b Histopathological features and the number of TUNEL-positive hepatocytes. The following values are presented as the mean + SD. Control chow, 0.03 ± 0.58; CDAA-HF-T(+), 1.20 ± 0.40; CDAA-HF-T(−), 3.03 ± 0.50 (c)

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