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Fig. 5 | Lipids in Health and Disease

Fig. 5

From: A trans fatty acid substitute enhanced development of liver proliferative lesions induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet

Fig. 5

SULT1E1 and IGF-1 expression levels. Two-dimensional heat map of the expression values of DEGs where arrows indicate the genes overexpressed only in the CDAA-HF-T(−) group (a). qPCR of the SULT1E1 gene The following values are presented as the mean + SD. Control chow, 1.14 ± 0.58; CDAA-HF-T(+), 4.65 ± 1.47; CDAA-HF-T(−), 10.20 ± 4.87 (b). Immunoblot analysis of the SULT1E1 protein. Densitometric outcomes of the immunoblot analyses of SULT1E1 levels expressed as the ratios versus GAPDH level. The following values are presented as the mean + SD. Control chow, 1.00 ± 0.03; CDAA-HF-T(+), 5.37 ± 1.73; CDAA-HF-T(−), 11.60 ± 3.57. ← indicates SULT1E1 band, stands for non-specific bands (c). qPCR of the IGF-1 gene at the end of weeks 13 and 26. The following values are presented as the mean + SD. IGF-1 gene at the end of week 13: control chow, 1.01 ± 0.13; CDAA-HF-T(+), 0.87 ± 0.07; CDAA-HF-T(−), 0.77 ± 0.17. IGF-1 gene at the end of week 26: control chow, 1.02 ± 0.23; CDAA-HF-T(+), 0.89 ± 0.30; CDAA-HF-T(−), 0.37 ± 0.17. d *Significantly different from the control value. +Significantly different from the CDAA-HF-T(+) value

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