Fig. 3
CIR signals identified binding of 504B3 with 4 different phospholipid forms. a-e CIR signal was plotted against the ligand concentrations to determine the binding constant (KD) for 504B3 anti-LPA antibody against five lysophospholipid forms. Binding curves for each ligand ((a) 18:1 LPA, (b) 16:0 LPA, (c)18:1 PA and (d)18:1 LPC and (e) all ligands) were plotted with the negative control S1P (no signal). Each data point represents multiple binding isotherms (experimental replicates) (18:1 LPA, N = 4; 16:0 LPA, N = 4; 18:1–18:1 PA, N = 2; 18:1 LPC, N = 4, and 18:1 S1P, N = 2), each with six to seven technical replicates