Fig. 3

The fibre-type specific metabolic profiles of 3630 muscle fibres from 17 male rats across four groups. The top, middle, and bottom rows present data for type I (a-c), type IIa (d-f), and type IIb myofibres (g-h) respectively. The columns from left to right present data on the percentage of high neutral-IMCL containing fibres (as assessed via Oil Red O staining) (a,d,g), the percentage of high-glycogen containing fibres (as assessed via glycogen periodic acid Schiff staining) (b,e,h), and finally the overall percentage of each row’s muscle fibre as a percentage of the total fibres quantified (fibre type assessed via metachromatic myosin ATPase staining) (c,f,i). The four groups are Control Sedentary (CS); diabetic with conventional insulin therapy (DCT); diabetic with intensive insulin therapy (DIT); and diabetic with combined exercise training and conventional insulin therapy (DARE). All data are expressed as mean ± SEM. * denotes 0.05 > P > 0.005; ** denotes P = 0.0047; *** denotes P = 0.0017; **** denotes P = 0.0003; ***** denotes P < 0.0001. Note that all changes in IMCL storage occurred within the type I oxidative fibres, and that there are a greater percentage of these type I myofibres in the DIT and DARE groups when compared to control and DCT