Fig. 5
IRS1 knockdown inhibits adipocyte differentiation and is regulated by miR-410-3P. a. qPCR analyses and Western Blot analyses of IRS-1 in groups NC and knockdown of IRS-1(#1 and #2). b. Oil Red O staining observed with 10X and 20X eyepiece in groups NC and knockdown of IRS-1(#1 and #2). c. Quantitative analysis of lipid accumulation and free fatty acids of cell supernatant in groups NC and knockdown of IRS-1(#1 and #2).d. qPCR analyses of C/EBP-a, C/EBP-β, and PPAR-γ in groups NC and knockdown of IRS-1(#1 and #2). e. Western Blot analyses of C/EBP-a, C/EBP-β and PPAR-γ in groups NC and knockdown of IRS-1(#1 and #2). f and g. qPCR analyses and Western Blot analyses of C/EBP-a, C/EBP-β and PPAR-γ in NC group and miR-410-3p mimics concentration gradient group. All data are expressed as mean ± SEM. * P < 0.05, ** P< 0.01, *** P < 0.001