Transcriptome condition | In vitro comparator | URG in sputum Mtb | DRG in sputum Mtb | Ref |
---|---|---|---|---|
SMtb vs Culture | 7H10 agar /7H9 broth/Dubosa | dosR, icl1, hspX, narK2, tgs1, PE/PPE | nuoB, qcrC, and ctaD | [29] |
SMtb Vs Culture | No information | Conserved Hypotheticals. mprAB: dosR is stable | pks15/1, pks10 Pks12, phoP, ESX 1-ESX-5 | [89] |
SMtb Vs Culture | Liquid culture | ACOD1/IRG1, GLUT1, MCT4, ESX-3, Rv0106, Rv2990c, ESX-3, mprA | TCA cycle, ETC, NADH dehydrogenase, pentose phosphate pathway (PPP), NAPDH, ROS. PhoP, small RNA mcr7, pks12, esat-6 and cfp-10, phoP | [91] |
SMtb Vs Culture | MGIT 460 | Glyoxylate shunt, methylcitrate cycle (icl, prpC and rv1129c), catabolism of cholesterol and fatty acids, and tgs; Nitrate reduction (narK2/3). dosR nrdZ, narK2, rv1738, pfkB, hspX, hrp1,rv3126c and rv3128c | gltA2, kgd, mdh, korA/B, sucC, rv0247c/48c, fumC and mqo), FAS-1 (fas), FAS-II, mmaA2/3/4, cmaA2, pcaA, fadD32 and pks13, NADH dehydrogenase, cytochrome C reductase | [92] |
SMtb Vs culture | 7H9/ DTAc | sputum and BAL had significant up-regulation of the dosR regulon | Ribosomal genes and primary metabolism genes | [93] |
Day 7–14 days Vs day0 | None applicable | Anaerobic respiration, PE/PPE genes, is, dosR,transcriptional factors, oxidative stress, sigma factors, toxin-antitoxin modules, | TCA cycle, ATP synthesis, ribosomal proteins, pks, ESX, replication, efflux pumps, drug-activating enzymes & drug targets | [94] |
SMtb at day 3/7/14 Vs day 0 Rx | None applicable | Mtb responses at 7 and 14 daysduring chemotherapy were most similar to that of bacillibefore drug therapy had begun | methylcitrate | [92] |
Day14 Vs day2 | None applicable | tgs, and ATP-binding cassette transporter and toxin. Rv1258c, bacA, and mmr, rpoB. TA modules, sigma factors | ESX and ribosomal genes, drug-activating enzymes katG, pncA, and ethA, gyrase, bedaquiline target atpE | [94] |
BAL vs sputum | 7H9/ DTAc | dosR regulon expression was higher in BAL than in sputum | BAL had lower expression of ribosome proteins | [93] |
Lipid-NRP1 Vs Dextrose-NRP1 | Dubos brothb | Higher virulence, detoxification & adaptation, lipid metabolism, intermediary metabolism& respiration, regulatory protein | Insertion sequences & phages | [60] |
In NRP-2 state | 7H9/ DTAc | dosR regulon, oxidative stress responses, anaerobic respiration | Growth and metabolism | [93] |