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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Palmitic acid causes increased dihydroceramide levels when desaturase expression is directly silenced or indirectly lowered by silencing AdipoR2

Fig. 2

Summary of all experiments. Each row name (left) represents a separate experiment in HEK293 cells (except for JLR_2019_HepG2 that was performed with HepG2 cells) with at least biological triplicates for each condition performed in either basal media (containing BSA only) or in the presence of palmitic acid (conjugated to BSA) and in the presence of the indicated (right) siRNAs or over-expression. Sphingolipids scored were: ceramides (Cer), dihydroceramides (DiCer), glucosylceramides (GluCer), lactosylceramides (LacCer) and sphingomyelins (SM). This sphingolipid data is previously unpublished though some of the experiments were performed as part of published work that included siRNA efficacy assays and phospholipid quantification: PLoS_Gen_2017 [23], JLR_2019 and JLR_2019_HepG2 [29], eLife_2018_1/2 [25], BBA_2021_1/2/3 [30]

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