Fig. 5

AdipoR2 silencing causes increased incorporation of palmitate into phosphatidylcholines, phosphatidylethanolamines and ceramide (data from d31PA100). (A) and (B) shows the levels of d31-PA incorporation in phosphatidylcholines (PC) and phosphatidylethanolamines (PE) of HEK293 cells grown in 100 μM d31-PA and treated with Non-target siRNA (NT), AdipoR1 siRNA or AdipoR2 siRNA. The sums of ceramides (Cer) and glucosylceramides (GluCer) species containing d31-PA (C-D) or unlabeled fatty acids (E-F) are shown as box plots while their fatty acid composition is shown as a heat map (G). In the box plots, boxes indicate the 25th to 75th percentile while the whiskers indicate the data points still within 1.5 of the box range. For A-F, significant differences from the NT siRNA control were determined using one-way ANOVA and post-hoc Dunnett tests, with *P < 0.05, **P < 0.01 and ***P < 0.001. For the heat map, the amount of each lipid species was normalized to the average of the NT + 100 μM d31PA treatment and the heat maps show fold differences from the mean across all treatments (each column is a replicate); only lipid species with significantly different levels among siRNAs in a given culture condition are included (ANOVA, q < 0.05)