Table 1 Primers for detection of possible off-target sites
Primers | off-targets of GCAGGGCCAGAGCCCAGGTG AGG |
|---|---|
off-targets-1 | 5′ TCGTCGGCAGCGTCACAAAGTGCTTACGGGCAGA 3’ 5′ GTCTCGTGGGCTCGGCTCCACTTTCCTCCCTGCAG 3’ |
off-targets-2 | 5′ TCGTCGGCAGCGTCGAGAAGAAGCACCACCCCTC 3’ 5′ GTCTCGTGGGCTCGGACAGGGATCAAGGAAGGACTG 3’ |
off-targets-3 | 5′ TCGTCGGCAGCGTCGGAAATGGTGAGTGAGCCCA 3’ 5′ GTCTCGTGGGCTCGGCAGTCACACGGCTTAGTCGT 3’ |
off-targets-4 | 5′ TCGTCGGCAGCGTCTCGTGAAGGACTCTCCACCA 3’ 5′ GTCTCGTGGGCTCGGAATCTCACCTTCGACAGCCG 3’ |
off-targets-5 | 5′ TCGTCGGCAGCGTCCGACAGGGGTGGGGAAAC 3’ 5′ GTCTCGTGGGCTCGGTTTCCCAGAAACCTGCCCTC 3’ |