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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Lipidomic analysis identifies age-disease-related changes and potential new biomarkers in brain-derived extracellular vesicles from metachromatic leukodystrophy mice

Fig. 4

Ceramides, Hexocylceramides and sulfatides are altered in brain derived EVs enriched from ARSA−/− mice. A) Graphical representation of enzymes involved in the synthesis of ceramides (Cer), Hexosylceramide (HexCer) and sulfatide (SHexCer). CGT: cerebroside galactosyl transferase; GALC: galactosylceramidase (also known as beta-galactocerebrosidase); CST: cerebroside sulfo transferase and ARSA: Arylsulfatase A. B-C) Volcano plots highlighting significantly (n = 3 per genotype and time point; p < 0.05) altered Cer, HexCer and SHexCer lipids observed in brain-derived EVs from MLD relative to control mice. Comparisons were done between lipidomic analysis of EVs from 30 postnatal days (P30), 3 months (3 m) and 6 months (6 m) of age in positive (B;top) and negative (C;bottom) ionization modes. Annotated are ceramide non-hydroxyfatty acid sphingone (Cer_NS, orange), ceramide alpha-hydroxy fatty acid-sphingosine (Cer_AS, dark blue), ceramide non-hydroxyfatty acid-dihydrosphingosine (Cer_NDS, red), hexosylceramide non- hydroxyfatty acid-phingosine (HexCer_NS,light blue),hexosylceramide alpha-hydroxy fatty acid-sphingosine (HexCer_AS, pink) and sulfatide (SHexCer, green). The key for number lipid species is presented in Table 1. n = 3 biological replicates per genotype and time point. Significance was determined via an unpaired t-test (p < 0.05, * p < 0.01, **; p < 0.001, ***)

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Lipids in Health and Disease

ISSN: 1476-511X