Fig. 2
From: Measures of high-density lipoprotein function in men and women with severe aortic stenosis
Characterization of human aortic vascular interstitial cells (HAVICs). (A, B) Marker for HAVICs in culture, alpha-smooth muscle actin (α-SMA), and ATP-Binding Cassette A1 (ABCA1) protein by immunofluorescence. Immunofluorescence staining with α-SMA (green) and ABCA1 (red) in (A) calcified and (B) non-calcified HAVICS are shown. Nuclei were stained (blue) with 4′,6-diamidino-2-phenylindole (DAPI). (C) ABCA1 protein expression in BHK cells was used as a control. Cells (100 μg) were separated by SDS-PAGE (8–28%) and probed for ABCA1 detection. (D) Data are expressed as the ratio of quantified band intensities for ABCA1 and GAPDH proteins