Fig. 4

Serum and hepatic levels of bile acids in adult male wildtype (WT), HNF4α heterozygote (HET), and HNF4α knockout (KO) mice fed 15 d with high-fat-high-sugar diet (HFHS) (N=5-6 per group). Bile acids were quantified by LC-MS/MS. A serum levels of CDCA; B serum levels of DCA; C serum levels of MCA; D serum levels of unamidated bile acids; E hepatic levels of T-MCA; F hepatic levels of T-HDCA; G hepatic ratio of %non-12α-OH: total bile acids; H hepatic hydrophobicity index (HI) of bile acids. In the box plots, the end of the lower whisker is the minimum value, the boundary of the box closest to zero indicates the 25th percentile, a black line within the box marks the median, the boundary of the box farthest from zero indicates the 75th percentile, and the end of the upper whisker is the maximum value. * P < 0.05 versus WT mice