Molecular effects of the consumption of margarine and butter varying in trans fat composition: a parallel human intervention study

Table 3 Delta change in serum levels of lipids [μg mL⁻¹ serum] after 4 week treatments. Only lipids that change significantly differently between treatments are shown. The Spearman’s correlation between median serum change in lipid per treatment group and content of lipids in the corresponding food products is represented by a letter code: +: weak positive (rho < 0.6); ++ moderate positive (rho 0.7–0.9); +++: strong positive (rho > 0.9). All correlations are positive

Fatty Acids	Median wTFA	IQR wTFA (Q1, Q3)	Median iTFA	IQR iTFA (Q1, Q3)	Median rTFA	IQR rTFA (Q1, Q3)	P_FDR-adjusted^#	sp
[μg mL⁻¹]	(n = 14)		(n = 14)		(n = 14)
SFA
C12:0	10^b	−15, 45	0^b	−10, 15	-60^a	−70, − 17.5	**	+
C15:0	0^a	−10, 10	-5^a	−17.5, 7.5	30^b	20, 40	***	++
C17:0	5^a	−10, 17.5	-5^a	−17.5, 10	35^b	30, 82.5	**	++
C18:0	30^a	− 368, 583	-125^a	−320, 300	660^b	190, 1783	*	+
12Me-C15:0	0^a	10, 0	0^a	−10, 0	10^b	10, 20	**	++
14Me-C16:0	0^a	−7.5, 10	0^a	0, 10	10^b	10, 20	**	++
15Me-C17:0	0^a	−7.5, 10	5^a	−10, 10	30^b	2.5, 50	*	++
PUFA cis
C20:4c	0^a	0, 10	-5^a	−10, 0	20^b	10, 35	**	++
C20:5c	15^a	0, 42.5	-10^a	−5, 37.5	65^b	30, 153	**	++
C22:5c	10^a	−10, 27.5	-10^a	−20, 15	60^b	32.5, 128	***	++
TFA
C16:1 t9	0^a	0, 10	0^a	−10, 10	25^b	10, 47.5	**	++
C18:1 t6 + t7 + t8 + t9	10^a	−17.5, 10	115^b	92.5, 185	10^a	−7.5, 20	**	+++
C18:1 t10 + t11	0^a	−10, 10	75^b	27.5, 90	115^c	95, 153	***	+++
C18:1 t12	0^a	0, 0	25^b	12.5, 30	10^b	0, 17.5	**	++
C18:1 t13 + c6 + c7	0^a	0, 10	30^b	12.5, 38	20^b	12.5, 30	**	+
C18:2u	0^a	−10, 7.5	0^a	−10, 0	10^b	10, 17.5	**	++
C18:2 t9 + c12	0^b	0, 7.5	-5^a	−10, 0	10^b	0, 10	**	+++
C18:2c9 + t11	0^a	−10, 7.5	0^a	−17.5, 7.5	80^b	52.5, 105	**	++
Sum parameters
Total C18:1 t	10^a	−7.5, 27.5	285^b	163, 335	155^b	103, 198	***	+
Total C18:2 t (with CLA)	-5^a	−17.5, 0	0^a	−27.5, 10	80^b	40, 123	***	++
Total CLA	−10^a	-10, 10	0^a	−17.5, 10	75^b	45, 115	***	++
Total TFA (without CLA)	20^a	−7.5, 47.5	275^b	115, 328	165^b	125, 225	***	+
Total TFA (with CLA)	20^a	−10, 62.5	290^b	77.5, 338	225^b	172.5, 337.5	***	+
Total ω-3 FA	90^ab	−40, 228	-15^a	−200, 135	260^b	102.5, 908	*	++

Legend: ^#Kruskal wallis test results (P_FDR-adjusted < 0.05: *, P_FDR-adjusted < 0.01: **, P_FDR-adjusted < 0.001: ***). Different letters (a, b, c) denote significant differences between treatment based on paired Wilcoxon signed-rank test (P_FDR-adjusted < 0.05)
Abbreviation: sp Spearman correlation, IQR Interquartile range. The FAs isomers that cannot be separated by the chromatographic steps are indicated by the plus sign “+” joining various cis (c) and/or trans (t) forms of the FAs

ISSN: 1476-511X