Fig. 5

CAAT-CM effect on BC cell morphological features, CSC, and EMT markers gene expression. Photomicrographs represent the morphological change of (A) MDA-MB-231 and (B) SUM-149 BC cells after treatment with 25 % CAAT-CM for 24 h. MDA-MB-231 cells showed elongated spindle-shaped morphology compared to untreated cells. In contrast, SUM-149 cell morphology clustered as aggregates. Cell morphology was observed by phase-contrast microscopy. Scale bar, 200 μm. (C) The mRNA expression of CD24 was significantly downregulated in the treated MDA231 with CAAT-CM compared with the control. D no change in the mRNA expression of CD44 was observed, E the mRNA expression of vimentin tended to be significantly upregulated in the treated MDA231 cells with CAAT-CM compared with the control by (3.08 fold), and F the mRNA expression of E-Cadherin was significantly downregulated in the treated MDA231 cells with CAAT-CM compared with the control. G The mRNA expression of CD24 tended to be significantly downregulated in the treated SUM149 cells, H No change in the mRNA expression of CD44 and I vimentin was observed, and J the mRNA expression of E-Cadherin was significantly upregulated in the treated SUM149 cells with CAAT-CM compared with the control by (2.38 fold). ^*P < 0.05 as determined by Student’s t-test. MDA-231 and SUM-149 cells were seeded in the absence (control) and presence of 25% CAAT-CM for 24 h (treated). Relative quantification of mRNA expression was analyzed using qRT-PCR (n of non-IBC = 18 and n of IBC = 9). Quantification of target genes mRNA was expressed relative to the housekeeping gene (18S) mRNA. The data shown are the mean ± SEM