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Fig. 5 | Lipids in Health and Disease

Fig. 5

From: FGF15 promotes hepatic NPC1L1 degradation in lithogenic diet-fed mice

Fig. 5

FGF15-FGFR4 pathway decreased biliary cholesterol reabsorption. Immunoblotting analysis was applied to assess the differences of hepatic NPC1L1 protein levels in mNPC1L1hepatic-OE mice fed with 8-week chow diet and HFCD with or without FGF15 treatment (A), as well as those fed with 8-week chow diet and LD with or without H3B-6527 treatment (B), with tubulin controls. The band densities of western blot images were analyzed with the ImageJ software and indicated below the bands, normalized to their loading controls. C The serum FGF15 concentrations in mNPC1L1hepatic-OE mice fed with 8-week chow diet and HFCD with or without FGF15 treatment, as well as those fed with 8-week chow diet and LD with or without H3B-6527 treatment. The (D) biliary cholesterol (a), bile acids (b), phospholipid (c) and (d) CSIs in a-d panels, E cholesterol (a) and triglyceride (b) from liver tissues, F cholesterol (a) and triglyceride (b) from plasma were from the mNPC1L1hepatic-OE mice fed with 8-week HFCD with or without FGF15 treatment and mNPC1L1hepatic-OE mice fed with 8-week LD with or without H3B-6527 treatment. Mann-Whitney U-test for data with non-normal distribution and Student’s t-test for normal distributions. *P < 0.05, **P < 0.01, ns no significance. HFCD, high fat-cholesterol diet; LD, lithogenic diet; CSI, cholesterol saturation index

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