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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Intervention time decides the status of autophagy, NLRP3 activity and apoptosis in macrophages induced by ox‐LDL

Fig. 2

Inhibition of ox-LDL phagocytosis in the early or advanced stage of atherogenesis is associated with a different autophagic status in macrophages. a Intracellular lipid content was measured by ORO staining after the cells were treated with cytochalasin D (3 μM) at 12 h and 36 h. Scale bar = 200 μm. b Western blot analyses of autophagy-related proteins after blockage of ox-LDL phagocytosis at 12 h or 36 h. c Macrophages were transfected with an adenovirus expressing mRFP-GFP-LC3 to measure autophagic flux intensity quantitatively. Scale bar = 20 μm. d Autophagosomes (indicated by the red arrows) were observed by TEM. Scale bar = 10 μm. *P < 0.05; **P < 0.01; ***P < 0.001

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Lipids in Health and Disease

ISSN: 1476-511X