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Fig. 5 | Lipids in Health and Disease

Fig. 5

From: Intervention time decides the status of autophagy, NLRP3 activity and apoptosis in macrophages induced by ox‐LDL

Fig. 5

Application of si-NLRP3 in the early or advanced stage of atherogenesis alters autophagic capacity in macrophages induced by ox‐LDL. a Western blot was utilized to analyze NLRP3 expression after exposure to si-NLRP3. b Western blot analyses of NLRP3 and autophagy-related proteins after si-NLRP3 exposure in the early and advanced stage. c Immunofluorescence was used to assess the expression of NLRP3 (green fluorescence). DAPI-stained nuclei (blue) are also shown. Scale bar = 100 μm. d Macrophages were transfected with an adenovirus expressing mRFP-GFP-LC3 to measure autophagic flux intensity. Scale bar = 20 μm. e Autophagosomes (indicated by the red arrows) were detected by TEM. Scale bar = 10 μm. f Intracellular lipid droplets were observed using ORO staining after si-NLRP3 was administered in the early and advanced stage. Scale bar = 200 μm. *P < 0.05; **P < 0.01; ***P < 0.001

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