Fig. 2

In vitro overexpression of circLDLR ameliorates the development of NAFLD by activating the autophagy signaling pathway. A ORO staining showed the lipid droplets in overexpressed circLDLR Hepa1-6 cells. B and C Hepa1-6 cells with or without OA/PA treatment to transfect with circLDLR overexpression plasmid, and an enzymatic method was used to measure intracellular TG and TC contents. *P < 0.05 vs. BSA group, ^#P < 0.05 vs. vector group. D Hepa1-6 cells with overexpressed circLDLR were transfected with Ad-LC3-GFP-RFP adenovirus and then assessed by immunofluorescence using confocal microscopy. The number of RFP- and GFP-LC3 puncta per cell were quantified and the amount of dots was calculated in the minimum of three independent visual fields from three separate wells. ^*P < 0.05, ^***P < 0.001 vs. vector group; ^##P < 0.01, ^###P < 0.001 vs. circLDLR OE group; &&P < 0.01 vs. vector + PA/OA group. E LC3 and p62, and LAMP2 expressions in Hepa1-6 cells were determined using western blot and relative quantification. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. vector group; ^###P < 0.001 vs. circLDLR OE group; ^&&P < 0.01, ^&&&P < 0.001 vs. vector + PA/OA group