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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Dietary fat induced chylomicron-mediated LPS translocation in a bicameral Caco-2cell model

Fig. 3

Effect of 1 week of apical FBS deprivation prior to digested palm oil and sunflower oil exposure on the intestinal integrity and basolateral ApoB secretion of Caco-2 cells. A Effect of digested palm oil and sunflower oil (both 1:50 diluted) on the intestinal integrity of Caco-2 cells, as assessed by TEER, preceded by 1 week of apical FBS deprivation. TEER is expressed as % compared to the control digest (also 1:50 diluted), which was set to 100%. Digested oils and control digest were diluted with DMEM w/o FBS and w/o phenol. Exposures were conducted as 3 biological replicates (N = 3). B Effect of digested palm oil and sunflower oil (both 1:50 diluted) on basolateral ApoB secretion by Caco-2 cells after 1 week of apical FBS deprivation. ApoB levels are expressed as relative levels compared to the control digest (also 1:50 diluted). Exposures were conducted in biological triplicate (N = 3); a, b, c represent significant differences between the treatments (P < 0.05)

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