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Fig. 5 | Lipids in Health and Disease

Fig. 5

From: Dietary fat induced chylomicron-mediated LPS translocation in a bicameral Caco-2cell model

Fig. 5

Effect of LPS concentrations on basolateral ApoB secretion and LPS translocation by Caco-2 cells after coexposure to digested palm oil and sunflower oil. Effect of different concentrations of LPS (ng/mL) on ApoB secretion (A) and LPS translocation (B) by fat-exposed Caco-2 cells. Digest control, digested palm oil and digested sunflower oil were diluted 1:50 in DMEM w/o phenol red and w/o FBS. Caco-2 cells were coincubated with LPS concentrations of 0, 100, 250, 500, 750, and 1000 ng/mL for 24 h. LPS was measured with the HEK-TLR4 assay. The OD represents the LPS concentration in the basolateral samples. Exposures were conducted as a single-cell experiment

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