Fig. 9
From: Dietary fat induced chylomicron-mediated LPS translocation in a bicameral Caco-2cell model
Chylomicron blockage by PL81 and its effect on basolateral ApoB secretion and LPS translocation. Effect of PL81 with or without palm oil and sunflower oil on Apo-B secretion (A) and LPS translocation (B) by Caco-2 cells after 1 week of apical FBS deprivation. Caco-2 cells were preincubated with 0 and 1000 ng/mL LPS overnight. Digest control, digested palm oil and digested sunflower oil were diluted 1:50 in DMEM w/o phenol red and w/o FBS with 500 µg/mL PL81. LPS was measured using the Pyrochrome® assay. These exposures were conducted as 3 biological replicates (N = 3) (P < 0.05)