Fig. 4
IL-8 in EVs induces lipolysis partly by activating NF-κB signaling pathway. A-C Western blot and the densitometric analysis of phosphorylated-p65, nuclear p65, total p65, UCP1, PGC1α in 3 T3-L1 adipocyte treated with IL-8, LLC-EVs (10 μg), C26-EVs (10 μg) and/or anti-IL-8 (n = 3), as indicated. D The activation of p65 NF-κB (as marked in Green) in 3 T3-L1 adipocytes was confocally imaged upon co-culturing with IL-8 (with and without specific anti-IL-8), LLC-EVs and/or C26-EVs (with and without specific anti-IL-8). The nucleus (Blue) was labeled with DAPI and 10 μg EVs were applied in test. E Oil red O staining assay for above groups. Bar = 100 μm. F Relative levels of glycerol release in cell supernatants upon above treatments. *P < 0.05, **P < 0.01, ***P < 0.001