Skip to main content
Fig. 5 | Lipids in Health and Disease

Fig. 5

From: Exosomal IL-8 derived from Lung Cancer and Colon Cancer cells induced adipocyte atrophy via NF-κB signaling pathway

Fig. 5

CXCR2 inhibitors partially relieved adipocyte wasting caused by IL-8 and EVs. A The viability of 3 T3-L1 adipocytes treated with different doses of CXCR2 inhibitor SB225002 as assessed by CCK8. B-D Western blot analyses of phosphorylated-p65, total p65, UCP1, and PGC1α in adipocytes co-cultured with IL-8 (with and without SB225002), LLC-EVs and/or C26-EVs (with and without SB225002) (n = 3), as indicated. 10 μg EVs were used in this experiment. E Oil red O staining test of the above groups. Bar =100 μm. F Relative levels of glycerol release in cell supernatants with the above treatment. 10 μg EVs were used in this experiment. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X