Table 1 Results of stability, accuracy, precision, recovery and matrix effect in quality control samples (n = 6)
Analyte | Sample | Added concentrations in QCs (ng/mL) | Matrix effect (%) | Stability (RSD, %) | Recovery (mean ± SD, %) | Intra- and Intraday accuracy (RE, %) | Intra- and Intraday precision (RSD, %) |
|---|---|---|---|---|---|---|---|
Sphinganine (d16:0) | Brain | 3 2000 4000 | 108.1 98.3 94.0 | 11.2 8.4 | L:83.6 ± 4.2 M:88.5 ± 3.6 H:82.0 ± 6.1 | 97.8–103.1 | 3.6–5.8 |
Blood | 3 1000 3000 | 102.9 96.8 106.8 | 4.1 4.3 | L:90.8 ± 4.9 M:82.7 ± 3.5 H:83.2 ± 7.6 | 97.3–99.9 | 1.4–11.9 | |
Cell | 3 2400 3600 | 98.0 93.8 110.4 | 5.8 9.8 | L:91.2 ± 5.8 M:92.8 ± 4.1 H:80.5 ± 7.4 | 98.7–100.9 | 3.3–7.6 | |
S1P | Brain | 10 200 400 | 98.9 99.6 98.2 | 10.9 4.7 | L:90.2 ± 2.1 M:88.3 ± 4.5 H:87.2 ± 1.6 | 94.5–109.6 | 5.4–8.3 |
Blood | 10 125 375 | 100.4 102.2 92.3 | 7.2 6.7 | L:83.4 ± 2.7 M:93.3 ± 4.1 H:91.5 ± 3.4 | 100.1–101.8 | 3.1–7.4 | |
Cell | 10 36 54 | 105.1 102.0 98.6 | 10.9 8.3 | L:102.4 ± 5.6 M:87.8 ± 2.4 H:94.1 ± 3.1 | 101.2–103.8 | 2.6–4.8 | |
Sphinganine (d18:0) | Brain | 3 8000 16,000 | 99.6 92.8 98.7 | 7.1 5.6 | L:85.1 ± 1.9 M:90.8 ± 2.0 H:83.7 ± 1.8 | 96.1–108.3 | 6.1–10.2 |
Blood | 3 5000 15,000 | 106.5 95.9 95.1 | 12.4 10.2 | L:91.4 ± 2.2 M:84.7 ± 4.6 H:96.6 ± 4.2 | 99.4–104.8 | 6.5–9.0 | |
Cell | 3 6000 9000 | 95.3 94.3 100.9 | 4.8 7.5 | L:83.1 ± 6.8 M:92.3 ± 1.4 H:87.2 ± 5.7 | 99.4–99.9 | 2.1–6.2 | |
Phytosphingosine | Brain | 3 4000 8000 | 105.7 105.4 93.2 | 7.8 8.1 | L:89.7 ± 3.6 M:91.7 ± 2.1 H:92.3 ± 4.0 | 92.3–107.4 | 4.6–9.1 |
Blood | 3 5000 15,000 | 100.4 95.7 94.1 | 4.6 7.0 | L:91.4 ± 1.2 M:83.4 ± 2.8 H:97.4 ± 6.4 | 98.7–103.9 | 2.1–11.8 | |
Cell | 3 3000 4500 | 101.7 103.5 100.9 | 9.6 5.9 | L:81.6 ± 2.3 M:91.8 ± 3.7 H:90.1 ± 5.4 | 97.9–99.3 | 2.8–4.9 |