Fig. 3

ATG2B regulates autophagy and pyroptosis in NASH models. A HepG2 telephone brought on by way of FFA apoptosis as detected through going with the flow cytometry assay. B Representative TEM photo (scale bar: 800 nm) of autophagosomes (indicated with the aid of purple arrows) in the NASH. C HepG2 cells are stained pink or vibrant red by way of hoechst 33,342/PI double staining (magnification, × 400) and the nucleus is no longer stained. Arrows point out PI-positive cells. Scale bar = 20 μm. D Immunofluorescence picture displaying LC3 and NLRP3 expression (red) in HepG2 cells brought about through FFA. E Western Blot showed autophagy and pyroptosis in the Rg1 and Rg1 + Rapa groups. F Release of LDH. *P < 0.05, **P < 0.01, ***P < 0.001