Fig. 6From: Exercise activates Sirt1-mediated Drp1 acetylation and inhibits hepatocyte apoptosis to improve nonalcoholic fatty liver diseaseSirt1 attenuates OA-induced lipid accumulation and mitochondrial dysfunction in HepG2 cells. A Oil red O staining for observing intracellular lipid accumulation; B immunofluorescence staining for checking intracellular ROS level; C Western blot for intracellular OXPHOS expression; D ATP production in cells was detected by kit; E mitochondrial membrane potential (ΔΨm) changes were detected by kit; F The copy number of mtDNA related to mitochondrial genesis was detected by kit; G Western blot for the level of NRF1 and TFAM; H: Immunofluorescence staining for the level of NRF1. (*P < 0.05, **P < 0.01, *** P < 0.001; ns: no significant difference)Back to article page