Dietary moderately oxidized oil activates the Nrf2 signaling pathway in the liver of pigs

Background Previous studies have shown that administration of oxidized oils increases gene expression and activities of various enzymes involved in xenobiotic metabolism and stress response in the liver of rats and guinea pigs. As these genes are controlled by nuclear factor erythroid-derived 2-like 2 (Nrf2), we investigated the hypothesis that feeding of oxidized fats causes an activation of that transcription factor in the liver which in turn activates the expression of antioxidant, cytoprotective and detoxifying genes. Methods Twenty four crossbred pigs were allocated to two groups of 12 pigs each and fed nutritionally adequate diets with either fresh rapeseed oil (fresh fat group) or oxidized rapeseed oil prepared by heating at a temperature of 175°C for 72 h (oxidized fat group). Results After 29 days of feeding, pigs of the oxidized fat group had a markedly increased nuclear concentration of the transcription factor Nrf2 and a higher activity of cellular superoxide dismutase and T4-UDP glucuronosyltransferase in liver than the fresh fat group (P < 0.05). In addition, transcript levels of antioxidant and phase II genes in liver, like superoxide dismutase 1, heme oxygenase 1, glutathione peroxidase 1, thioredoxin reductase 1, microsomal glutathione-S-transferase 1, UDP glucuronosyltransferase 1A1 and NAD(P)H:quinone oxidoreductase 1 in the liver were higher in the oxidized fat group than in the fresh fat group (P < 0.05). Moreover, pigs of the oxidized fat group had an increased hepatic nuclear concentration of the transcription factor NF-κB which is also an important transcription factor mediating cellular stress response. Conclusion The present study shows for the first time that administration of an oxidized fat activates the Nrf2 in the liver of pigs which likely reflects an adaptive mechanism to prevent cellular oxidative damage. Activation of the NF-κB pathway might also contribute to this effect of oxidized fat.


Background
In recent years, the contribution of oxidized fats to total energy intake has markedly increased in industrialized countries due to the rising consumption of deep-fried products [1]. In fast food restaurants, foodstuffs are typically fried in fats in fryers at temperatures close to 180°C. During the frying process, several chemical reactions occur within the frying oil resulting in the formation of a mixture of chemically distinct lipid peroxidation products [2]. Large quantities of the frying oil are absorbed into the fried food during deep-frying and therefore ingested during their consumption.
Feeding experiments with animals revealed that ingestion of oxidized fats provokes a wide array of biological effects [3][4][5]. One of the most striking effects of oxidized fat is the induction of oxidative stress which is due to lipid hydroperoxides absorbed from the ingested oxidized fats and reactive oxygen species (ROS) generated from microsomal cytochrome P450 enzymes which are induced by oxidized fat [6][7][8]. Oxidative stress in animals fed oxidized fats is evident by elevated concentrations of lipid peroxidation products, reduced concentrations of exogenous and endogenous antioxidants, and a decreased ratio of reduced and oxidized glutathione in plasma and tissues [8][9][10][11][12][13].
Previous studies have shown that administration of oxidized oils increases gene expression and activities of various enzymes involved in xenobiotic metabolism and stress response in the liver of rats and guinea pigs [14][15][16][17][18]. Hepatic xenobiotic metabolism and stress response is mainly controlled by nuclear factor erythroid-derived 2-like 2 (Nrf2), a master transcription factor shown to regulate more than 200 genes, including those involved in phase II detoxification and antioxidant defense [19]. Nrf2 pathway is regarded as the most important pathway in the cell to protect cells against oxidative stress [20,21]. Thus, the regulation of Nrf2 activity represents a critical step in initiating a cellular antioxidant response to ROS. It has been shown that oxidation products of n-3 fatty acids are able to activate Nrf2 in a human liver cell line, and thus to induce the expression of Nrf2 target genes involved in cellular defense [22]. More recently, we found that the ingestion of a dietary oxidized fat activates Nrf2 pathway in the intestinal mucosa of mice [23]. Based on these findings, it is likely that the induction of xenobiotic metabolism in the liver of animals fed an oxidized fat is due to an activation of Nrf2, which however has not yet been investigated. Therefore, the present study was performed to investigate the hypothesis that administration of an oxidized oil leads to an activation of Nrf2 pathway in the liver. For this end, we performed an experiment with pigs, an animal model which is closer to human physiology with respect to xenobiotic metabolism than rodents which are commonly used for the investigation of the biological effects of oxidized fats [24]. In order to reflect the practical situation of deep frying of foods in human nutrition, we used rapeseed oil-an oil commonly used for deep frying of foods-as source of fat which was heated at a temperature of 175°C for 72 h. To detect a potential activation of Nrf2 in the liver, we determined nuclear concentrations of Nrf2 and transcript levels of various Nrf2-regulated genes involved in phase II metabolism or antioxidant defense in the liver of pigs. In order to investigate whether changes in mRNA concentrations of Nrf2 target genes are reflected by altered activities, we determined activities of two Nrf2 target genes, superoxide dismutase (SOD) and thyroxine UDPglucuronosyltransferase (T4-UGT) in the liver. As an influence on the activity of T4-UGT affects the degradation of thyroxine, we also determined the concentration of that thyroid hormone in plasma of the pigs.

Animals and diets
For the experiment, 24 (12 male, 12 female) six week old crossbred pigs [(German Landrace × Duroc) × Pietrain] were used. The animals were kept in a pigpen controlled for temperature (23 ± 2°C), relative humidity (50-60%), and light from 06.00 to 19.00. After one week of adaptation, the pigs were weighed and randomly allocated to two groups, each consisting of 6 male and 6 female pigs, with similar average body weights. All pigs were housed in pairs in flat-deck pens and received a nutritionally adequate diet ( Table 1). The diets contained 16.4 MJ metabolizable energy and 224 g crude protein per kg. The two experimental diets varied in the type of fat. The diet of the treatment group ("oxidized fat group"), contained rapeseed oil (obtained from a local supermarket) which was heated in a domestic fryer (GF-8SE, Bartscher, Salzkotten, Germany) at a temperature of 175°C for 72 h, without addition of any foodstuffs. The diet of the control group ("fresh fat group") contained a mixture of fresh rapeseed oil and fresh palm oil (91.6:8.4, w/w). This fat mixture was used in order to equalize the fatty acid composition of the two oils (since the heating process caused a partial loss of polyunsaturated fatty acids (PUFA) in the rapeseed oil). Because the frying process caused a loss of tocopherols in the rapeseed oil, the native concentrations of tocopherols of all experimental fats were analysed. Based on the native concentrations of the fats, the vitamin E concentration of the oxidized fat was adjusted to that of the fresh fat by supplementation with all-rac-α-tocopheryl acetate (the biopotency of all-rac-α-tocopheryl acetate is considered to be 67% of that of α-tocopherol). The vitamin E concentration in the fresh fat diet was 97.6 mg α-tocopherol per kg diet, and that of the oxidized fat diet was adjusted accordingly. In order to avoid potential differences in food intake, due to adverse sensoric properties of the oxidized fat, a controlled feeding system was applied in which pigs were offered the same amount of diet during the 29 days feeding period. Water was available ad libitum from nipple drinkers during the entire experiment. All experimental procedures described followed established guidelines for the care and handling of laboratory animals and were approved by the local Animal Care and Use Committee (Regierungspräsidium Giessen; permission no: GI 19/3 No. 49/2010).

Sample collection
After completion of the feeding period the animals were anaesthesised and exsanguinated 2.5 h after their last meal. Blood was collected into heparinized polyethylene tubes and plasma was subsequently obtained by centrifugation of the blood (1100 × g, 10 min, 4°C). Tissue samples from liver were dissected and stored at -80°C until analysis.

Lipid analysis
The fatty acid composition of the dietary fats was determined by gas chromatography after methylation of fatty acids by trimethylsulfonium hydroxide [25]. The extent of lipid peroxidation of the experimental fats before inclusion into the diets was determined by assaying the peroxide value [26], thiobarbituric acid substances (TBARS) [26] and the percentage of total polar compounds [27].

RNA isolation and quantitative RT-PCR (qPCR)
For the determination of mRNA expression levels total RNA was isolated from liver tissue using Trizol™ reagent (Invitrogen, Karlsruhe, Germany) according to the manufacturer's protocol. Determination of total RNA concentration and purity, cDNA synthesis and qPCR analysis were performed as described recently in detail [28]. Gene-specific primer pairs obtained from Eurofins MWG Operon were designed using Primer3 and BLAST. Features of primer pairs are listed in Table 2. For determination of relative expression levels relative quantities were calculated using GeNorm normalization factor. In order to calculate the normalization factor, all C tvalues were transformed into relative quantification data by using the 2 -ΔCt equation, and the highest relative quantities for each gene were set to 1. From these values the normalization factor was calculated as the geometric mean of expression data of the three most stable out of five tested potential reference genes ( Table 2). Reference gene stability across samples was determined by performing GeNorm analysis [29]. After normalization of gene expression data using the calculated GeNorm normalization factor, means and SD were calculated from normalized expression data for samples of the same treatment group. The mean of the FF group was set to 1 and mean and SD of the OF group were scaled proportionally. Data on qPCR performance for each gene measured in liver are also shown in Table 2.

Immunoblot analysis
Nuclear extracts from liver were prepared from six animals per group with a Nuclear Extract Kit (Active Motif, Rixensart, Belgium) according to the manufacturer's protocol. Protein concentrations in the nuclear extracts were determined by the bicinchoninic acid (BCA) protein assay kit (Interchim, Montluçon, France) with BSA as standard. From each sample of nuclear extract, 30 μg protein were separated on 12.5% SDS-PAGE and electro-transferred to a nitrocellulose membrane (Pall, Pensacola, FL, USA). Loading of equal amounts of protein in each line was verified by Ponceau S (Carl Roth, Karlsruhe, Germany) staining. After incubation the membranes overnight at 4°C in blocking solution, membranes were incubated with primary antibodies against Nrf2 (polyclonal anti-Nrf2 antibody; Abcam, Cambridge, UK), NF-B/p50 (polyclonal anti-NF-B/p50 antibody; Santa Cruz, Heidelberg, Germany) and Histone H1 (polyclonal anti-Histone H1 antibody; Active Motif, La Hulpe, Belgium) as a nuclear reference protein to control for adequate normalization at room temperature. The membranes were washed, and then incubated with a horseradish peroxidise-conjugated secondary polyclonal anti-rabbit-IgG antibody (Sigma-Aldrich, Steinheim, Germany) for Nrf2, NF-B/p50 and Histone H1, respectively, at room temperature. Afterwards, blots were developed using ECL Advance (GE Healthcare, München, Germany). The signal intensities of specific bands were detected with a Bio-Imaging system (Syngene, Cambridge, UK) and quantified using Syngene GeneTools software (nonlinear dynamics).

Tocopherol concentrations
Concentrations of tocopherols in experimental diets, liver and plasma were determined by high performanceliquid chromatography (HPLC) with fluorescence detection [30,31]. Tocopherol esters in the samples were saponified with sodium hydroxide. Tocopherols were extracted with n-hexane, separated isocratically on a C-18-reversed phase column (Purospher 100 RP-18; Merck-Hitachi, Darmstadt, Germany), using methanol as mobile phase and detected by fluorescence (excitation wavelength: 295 nm, emission wavelength: 325 nm).

SOD activity
Activity of hepatic SOD was determined according to the method of Marklund and Marklund [32]. SOD activity in the homogenates was related to the protein concentration in the homogenates as determined by the BCA protein assay kit. One unit of SOD activity is defined as the amount of enzyme required to inhibit the autoxidation of pyrogallol by 50%.

Activity of T4-UGT and plasma thyroxine concentration
The activity of T4-UGT in the liver was assayed as described by Gessner et al. [33]. In brief, hepatic microsomes were incubated in a reaction mixture containing 1 μM thyroxine (T4), 0.1 μCi of 125 I-labeled T4, 75 mM Tris-hydrochloride (pH 7.8), 7.5 mM magnesium chloride, 5 mM UDP-glucuronic acid, 1 mM 6-propyl-2-thiouracil and 0.5 mg of microsomal protein/mL. T4 glucuronides formed during the incubation were separated from T4 and collected by HPLC. The radioactivity of the T4 glucuronide fraction as a measure of enzyme activity was counted with an automatic gamma counter (Wallac Wizard 3, Perkin Elmer, Rodgau, Germany). One unit of T4-UGT activity is defined as one pmol of T4 glucuronide formed per min. Plasma concentration of total T4 was measured with radioimmunoassay kits (MP Biomedicals, Eschwege, Germany).

Statistical analysis
Treatment effects were analyzed with one-way ANOVA using the Minitab Statistical software Rel. 13.0 (Minitab, State college, PA, USA). Statistical significance of differences of the mean values of the two groups of pigs was evaluated using Student's t test. Means were considered significantly different at P < 0.05.

Growth performance
The food intake of the pigs was identical for both groups due to the controlled feeding system applied ( Table 4). Initial body weights, daily body weight gains, feed conversion ratio and final body weights of the pigs did not differ between both groups (Table 4).

Tocopherol concentrations in plasma and liver
In order to assess effects of the oxidized fat on the antioxidative status of the animals, we determined concentrations of tocopherol in plasma and liver. Concentrations of α-tocopherol in plasma and liver -both, on absolute terms and expressed per mol of lipids-were reduced in the oxidized fat group compared to the fresh fat group (P < 0.05; Table 4). Concentrations of other tocopherols, beside α-tocopherol, in plasma and liver were negligible in both groups.

Nuclear concentration of Nrf2 in the liver
Activation of Nrf2 causes a translocation from cytosol into the nucleus. Therefore, the concentration of Nrf2 in the nuclear fraction was determined to evaluate activation of Nrf2 by the oxidized fat. Pigs receiving the oxidized fat had 4.6-fold higher nuclear concentrations of Nrf2 in the liver than pigs receiving the fresh fat (P < 0.05; Figure 1).

Nuclear concentration of NF-B in the liver
To further study whether other oxidative stress-sensitive transcription factors than Nrf2 are activated by the oxidized fat, we determined the concentration of the p50   subunit of NF-B in the nuclear fraction of the liver homogenates. Pigs receiving the oxidized fat had 2.2fold higher nuclear concentrations of NF-B/p50 in the liver than pigs receiving the fresh fat (P < 0.05; Figure 3).

Activities of SOD and T4-UGT in the liver
Pigs fed the oxidized fat had higher activities of SOD and T4-UGT in the liver than pigs fed the fresh fat (SOD: oxidized fat group, 105 ± 25 U/mg protein; fresh fat group, 75 ± 36 U/mg protein; T4-UGT: oxidized fat group, 4.4 ± 0.4 U/mg protein; fresh fat group, 3.8 ± 0.3 U/mg protein; P < 0.05 for both enzymes).

Concentration of thyroxine in plasma
In order to assess whether an increased activity of T4-UGT in the liver was leading to an alteration of thyroxine status, we determined the concentration of total thyroxine in plasma which was lower in pigs fed the oxidized fat than in pigs fed the fresh fat (oxidized fat group: 57.9 ± 14.6 nmol/L; fresh fat group: 72.7 ± 16.9 nmol/L; P < 0.05).

Discussion
The aim of this study was to investigate the hypothesis that consumption of a dietary oxidized fat leads to an activation of Nrf2 in the liver which in turn induces expression of genes involved in antioxidant defense and phase II metabolism. As a source of oxidized fat, we used rapeseed oil heated in a domestic fryer under practical conditions. Rapeseed oil is widely used for frying of foods in households and restaurants due to some advantages of partially hydrogenated fats. First, it has a more favourable fatty acid composition with respect to human health, particularly a favourable balance of n-3 to n-6 PUFA and a lower saturated fatty acid content. Second, it has the advantage that is must not be melted prior to use. Critical evaluation of the concentrations of lipid peroxidation products (peroxides, TBARS, polar compounds) indicates that oxidation of PUFA in the oil during heating occurred. Nevertheless, concentrations of these lipid peroxidation products were lower than in most other studies dealing with the effects of oxidized fats [34][35][36]. Although the parameters determined to assess the degree of oxidation such as TBARS are not very specific, the oil used in this study can be regarded as moderately oxidized. On the base of analyses of a large number of used frying fats, it has been found that heated fats are acceptable until they reach a level of 25% polar compounds [37]. Thus, the oxidized fat used in this study, containing 23% of polar compounds, would be just acceptable for frying of foods.
Heating an oil leads to a loss of its PUFA and its native antioxidants such as tocopherols [2,38]. In order to avoid secondary effects on metabolism due to differences in the intake of PUFA and antioxidants between the two groups, we equalized the control fat and the oxidized fat for their fatty acid compositions and their vitamin E concentrations. The vitamin E concentrations of the diets, being around 90 mg α-tocopherol equivalents per kg diet, were in excess of the vitamin E requirement which might be around 40 mg α-tocopherol equivalents per kg for the specific diets used in this study (according to calculations of the vitamin E requirement as a function of amount and type of PUFA as suggested by Muggli [39]).
In most studies dealing with the effects of thermoxidized fats, feeding the diet containing the oxidized fat lowered body weight gains in the experimental animals due to a diminished feed intake, a reduced digestibility of nutrients and general toxic effects [34,35,[40][41][42]. In the present study, feeding the diet containing the oxidized fat did not influence the growth of the pigs. This might be due to the facts that feed intake was equalized between the two groups of pigs by a controlled feeding system and that the heated oil was only moderately oxidized. The finding that the body weight development was not affected by the oxidized fat is advantageous from a methodological viewpoint because the effects of the oxidized fat were not confounded by secondary effects of reduced growth. Recent studies have shown that consumption of oxidized fats leads to a reduction of tocopherol concentrations in animal tissues due to a reduced digestibility and an enhanced turnover of vitamin E [8,11,12,43]. The finding of reduced α-tocopherol concentrations in plasma and liver of pigs fed the oxidized fat in the present study indicates that even moderately oxidized fats compromise tissue vitamin E status in animals.
According to the hypothesis of this study, we observed for the first time that administration of an oxidized fat leads to an activation of the transcription factor Nrf2 in the liver. Activation of Nrf2 was evident by an increased concentration of nuclear Nrf2 and increased transcript levels of several Nrf2 target genes involved in the antioxidant defense system (SOD1, GPX1, TXNR1, HO-1) and phase II metabolism (NQO1, MGST1, UGT1A1). Under normal conditions, Nrf2 is located in the cytosol of cells where it is constantly degraded via the proteasomal system. In response to various stimuli like oxidative stress, Keap1-a cytosolic inhibitory protein-dissociates from Nrf2 and Nrf2 translocates into the nucleus, where it binds to specific DNA-sequences called antioxidant response element (ARE) in the regulatory region of target genes [44]. Regarding that Nrf2 is activated by ROS [45,46], the occurrence of oxidative stress commonly observed in animals fed oxidized fats might be one possible explanation for activation of Nrf2 in pigs fed the oxidized fat. This possibility is strengthened by the observation that the up-regulation of enzymes involved in xenobiotic metabolism and stress response due to feeding of oxidized fats was attenuated by concomitant supplementation of vitamin C or vitamin E in guinea pigs and rats, resp [15,16]. Recently, it has been found that n-3 fatty acid oxidation products deriving from eicosapentaenoic or docosahexaenoic acid are able to directly activate Nrf2 by initiating dissociation of Keap1 [22]. Therefore, the possibility that Nrf2 activation was directly induced by specific oxidation products present in the oxidized oil cannot be excluded.
Some of the genes determined in this study, such as HO-1, GPX1 and SOD1, also contain binding sites for other oxidative stress-sensitive transcription factors, such as NF-B, in their regulatory region [47][48][49]. Therefore, it is likely that the up-regulation of those genes was at least in part mediated by activation of NF-B observed in the pigs fed the oxidized fat, as indicated by an increased nuclear concentration of p50. Thus, it is very likely that a coordinated activation of multiple oxidative stress-sensitive signaling pathways is responsible for the observed up-regulation of antioxidant, cytoprotective and detoxifying genes in the liver of pigs fed the oxidized fat diet, particularly because ROS, whose formation is induced by the administration of oxidized fats, are common stimuli of these pathways.
A further interesting observation of this study is that hepatic activity of T4-UGT, an enzyme which belongs to the phase II system, in the liver was increased in pigs fed the oxidized fat. T4-UGT catalyses the glucuronidation of thyroxine, and thus is the key enzyme of its elimination in the liver [50]. The finding of an increased activity of that enzyme concurs with a reduced concentration of total thyroxine in plasma of pigs fed the oxidized fat suggesting that hepatic elimination of that hormone was enhanced in those pigs. Thus, the present study indicates that oxidized fats could affect thyroid hormone status via an induction of the thyroid hormone elimination process in the liver such as observed for several other compounds which are acting as inducers of the hepatic xenobiotic system [51][52][53]. It is well known that hepatic phase I and phase II enzymes play an important role in elimination and detoxification of many drugs [54,55]. Thus, it is likely that ingestion of oxidized fats enhances the elimination of medical drugs, due to an induction of the hepatic xenobiotic system.
In conclusion, the present study shows for the first time that the ingestion of a moderately oxidized oil causes an activation of Nrf2 in the liver of pigs. This probably provides an explanation for the concomitant up-regulation of hepatic genes involved in the antioxidant defense system and phase II metabolism as observed herein in pigs and in recent studies in rats and guinea pigs [14][15][16][17][18][19]. Induction of Nrf2 in the liver of pigs fed an oxidized fat can be interpreted as an adaptive response of the liver to cope with oxidative stress induced by administration of oxidized fats, thereby, preventing ROSmediated damage. Activation of Nrf2 is generally regarded as a beneficial effect as it causes an up-regulation of a wide spectrum of antioxidant, cytoprotective and detoxifying genes and thus protects the cell against ROS and toxic compounds. Indeed, the coordinated upregulation of these genes by Nrf2 activators is considered as a potential therapeutic strategy to protect against insults such as inflammation and oxidative stress induced in various chronic diseases [56]. Nevertheless, the results of the present study must not be interpreted in the way that oxidized fats can be regarded as health-promoting components of the diet, as components of oxidized fats might have several adverse effects in human subjects. The results of this study rather suggest that oxidized fats are a mixture of chemically distinct substances, some of which exhibit a significant biological activity.