Acute stress show great influences on liver function and the expression of hepatic genes associated with lipid metabolism in rats

Background The theory of Chinese medicine believes rage harms normal liver function, namely ’raged impairing liver' in short. The purpose of this study is to investigate the impact of acute stress on liver lipid metabolism in rats. Methods and results Comparison of liver function indicators, serum lipid level of rats under acute stress and normal rats, as well as detection of liver tissue in the SR - BI, ABCG5 and ABCG8 protein and gene expression changes. Acute stressed rats had shown a lower serum levels of albumin (P<0.01), HDL- cholesterol (P<0.01) than normal rats, with higher serum levels of globulin (P<0.01) and LDL-cholesterol (P<0.05). Acute stressed rat’s liver tissue exhibited a lower protein expression of ABCG5 (P<0.05), ABCG8 (P<0.01) and a higher level of SR-BI (P<0.05), compared with to normal rats. Furthermore, liver gene expression of ABCG5 (P<0.01) and ABCG8 (P<0.05) were lower in acute stressed rats than in normal rats, while SR-BI was higher in acute stressed rats than in normal rats (P<0.01). Conclusions Acute stress had a direct influence on rat’s liver lipid metabolism.


Background
The word 'stress' first appeared in the index of Psychological Abstracts in 1944 [1]. Recently, stress has shown to play an important role in the onset of cardiovascular diseases, immunological disorders and pathophysiological consequences of normal aging [2][3][4]. Stress had also been shown that will inhibit metabolic function, such as lipid elimination and glycometabolism [5,6]. The theory Chinese Medicine believes rage harms liver function [7]. Meanwhile, acute stress causes rage, so acute stress may individually damages the liver. There are reports [8][9][10][11] that acute stress increases oxidation and diminishes antioxidant's protection, stating acute stress oxidates and destroys the liver. The liver is a crucial organ that facilitate lipid metabolism, including cholesterol metabolism and it is meaningful to study acute stress's effect on lipid metabolism in rats. In recent years, the study of lipid metabolism focused on reverse cholesterol transport (RCT), which means excess cholesterol transport from peripheral cells of apolipoprotein to the liver by high density lipoprotein (HDL), where lipid uptake took place and cleared. ATP-binding cassette transporter (ABC) G5, G8 and scavenger receptor-BI (SR-BI) are principal mediators of cholesterol efflux [12][13][14]. ABCG5 and ABCG8 mediate cholesterol outflow from peripheral cells, while SR-BI mediate hepatic selective uptake of cholesterol esters. Present study aims to discuss the effect of acute stress on lipid metabolism in rats from the perspective of reverse cholesterol transport.

Animals
Adult male Sprague Dawley rats weighing 160-180 g were obtained from the Central Animal Facility, Guangzhou University of Chinese Medicine (SCXK (Guangdong) 2008-0020), the rats were housed individually per cage upon arrival, they were allowed to adjust to their new environment in 1 week. The rat had free access to water and food, temperature of 25 ± 1°C, a 12-hour light-dark cycle (lights on at 08:00-20:00). Protocols were conducted in accordance with standard ethical guidelines, it was approved by the institution animal ethics committee (Grants 81102530/H2702 from National Natural Science Foundation of China). The rats were randomly selected into normal group and acute stress group (n = 8 in each group). Rats in the acute stress group were subjected to tail clipping at one third towards the end of the tail, three times a day for 30 minutes each time [15]. The normal control group were housed in a separate room and were not subjected to any stress for 14 consecutive days. Collection of blood, sacrifice of rats and liver isolation were performed after the restraint stress procedure.

Sucrose consumption test
All rats were given 1% sucrose solution for a 24 hour period in their cages with no food and water following food and water deprivation for 24 hours. Sucrose consumption was recorded by re-weighing bottles of solution.

Open-field test
Open-field test was conducted as described by Dunn [16], to assess acute stress effects on rats, the test was performed on the last 2 days before the end of the experiment. The apparatus consists of a rectangular box (80 × 80 × 50 cm), a floor divided into 25 (16 × 16 cm) rectangular units. The rats were placed in the center of an open field and were allowed to freely explore the area for 3 minutes. Two motor parameters were quantified throughout this test: locomotion frequency (numbers of with which the rat crossed one of the grid lines with all four paws) and rearing frequency (times of rearing with hind legs). The animals were acclimated to the experimental room for at least 2 hours prior to the beginning of the open-field test. Open-field tests were carried out in soundproof rooms without any human interference. The open-field was cleaned with a 5% water-ethanol solution before behavioral testing to eliminate bias due to odors left by previous rats. The evaluation of rat's motivation behavior was performed in a blinded manner by two independent observers. After testing inter-observer reliability, the mean from the results was statistically analyzed.

Quantitative real-time PCR
Total RNA was extracted with Trizol reagent (Invitrogen, Shanghai, China). The quality of samples was confirmed by agarose gel electrophoresis. Total RNA was reverse transcribed to complementary DNA using Prime Script® 1st Strand cDNA Synthesis Kit (TAKARA, Kyoto, Japan). The thermo cycler settings were 30°C for 10 minutes, 42°C for 60 minutes, and 70°C for 15 minutes. Real-time PCR was performed in triplicates using SYBR® Premix Ex Taq kit (TAKARA) in a 25 μL reaction volume. GAPDH was used as the housekeeping gene. The primers used for real-time PCR were listed in Table 1. Relative mRNA levels were calculated by the method of 2 -△△ Ct .

Statistical analysis
Each parameter was expressed as the mean ± SEM. Statistical significance was determined by t-Student's test. A p value of less than 0.05 was considered significant.

Sucrose consumption test
Compared with the activity of sucrose consumption of normal group, 2.36 ± 0.15, the activity of sucrose consumption  (Figure 1).

Acute stress exposure resulted in depression-like symptoms
Compared to the activities of the horizontal and the vertical of normal group, which 84 ± 9 and 20 ± 4, the activities of the horizontal and the vertical of model group were much lower, the results of these two indexes were 56 ± 15 and 13 ± 5. We can find remarkable statistical difference (P<0.01 or P<0.05) (Figure 2).

Body weight and liver coefficient
As shown in Figure 3A, during the control phase (baseline), all animals (normal and model groups) exhibited a similar weight, 180.7 ± 8.1 g and 178.1 ± 10.0 g. However, after two weeks of acute stress, reduced this preference in the model group, which are 183.0 ± 8.6 g and 202.2 ± 14.5 g, the body weight of normal group in the next were 229.7 ± 8.5 g and 256.8 ± 14.7 g, the differences between two groups extremely remarkable difference (P<0.01) ( Figure 3A). As shown in Figure 3B, two weeks of acute stress decrease the relative liver coefficient (weight/100 g body weight), 3.16 ± 0.12, the liver coefficient of normal group, 3.43 ± 0.18, is much higher as compared with the liver coefficient of model group, which states notable statistic difference (P<0.01) ( Figure 3B).

Comparison of serum and blood lipid metabolism index between each group
The level of TC, TG, LDL-C and HDL-C of the normal rats were 1.56 ± 0.14 mmol/L, 0.46 ± 0.14 mmol/L, 0.22 ± 0.03 mmol/L, 0.73 ± 0.06 mmol/L, comparing to the normal group, the rats have a higher level of TC, 1.61 ± 0.12 mmol/L, higher level of TG, 0.47 ± 0.14 mmol/L, but shown no statistical differences (P>0.05), a clear higher level of LDL-C, 0.30 ± 0.07 mmol/L, a lower level of HDL-C at 0.61 ± 0.05 mmol/L, which show remarkable statistic differences (P < 0.05 or P < 0.01) ( Table 2).

Comparison of serum liver function index between each group
Compared with the activities of ALB, GLB, ALT and AST of normal group, which are 38.9 ±0.8 g/L, 21.3 ± 1.1 g/L, 43 ± 12 U/L and 97 ± 15 U/L, respectively, the rats in model group has a lower level of ALB, 34.8 ± 2.8 g/L, a higher level of GLB, 23.0 ± 1.0 g/L, the difference between these two groups were statistically significant (P < 0.01). The level of ALT and AST of the rats in model group were separate at 41 ± 10 U/L and 105 ± 29 U/L, comparing to the index of normal group, it illustrate no statistical differences (P>0.05) ( Table 3).

Acute stress affects lipid metabolism in rats
We examined the effects of acute stress on expression levels of liver ABCG5, ABCG8 and SR-BI using western blotting. As shown in Figure 4B, the expressions of ABCG5 and ABCG8 of all of these livers were lower in model rats, while SR-BI was higher. These results were confirmed by quantitative PCR assays showing that messenger RNA (mRNA) levels of ABCG5 and ABCG8 were all down-regulated in model rats ( Figure 4A) whereas SR-BI mRNA seemed to be up-regulated.

Discussion
Anger is one of the seven emotions in Traditional Chinese medicine (TCM). Chinese medicine theory believes raged harms normal liver function, 'raged impairing liver'. SU WEN · The disease theory, wrote "In great anger, an increase in Qi damages the liver." Meanwhile, SU WEN · Theory of Yin and Yang, wrote "anger, as liver emotion", the so-called anger is metaplasia by the vital essence. If anger hurt viscera, it hurts the born of the viscera. Stress was put forward by Han Salye in 1944, it is a kind of syndrome reaction state including nerve, endocrinology and immunity, when the body faces strong stimulus from the outside world. When confronting stressors, the hypothalamic-pituitary-adrenal axis is excited during stress, the adrenal endocrine releases more glucocorticoids, meanwhile, the glucose level in blood is also higher, and the immunity function is depressed. Good stress reaction is beneficial to the body in a short term environment, if the body under stress for an extended time, the HPA axis is over excited, GC level is too high, then the body is hurt. Stress has been shown to play an important role in the onset of  cardiovascular disease [17][18][19][20], it has also been found to inhibit metabolic functions such as lipid elimination and glycometabolism [5,6].
There have studies [8][9][10][11] reported that stress has some influences on the liver, such as oxidative stress. According to Chinese medicine, rage harms the liver, since liver plays a great role in lipid metabolism, studying the impact of acute stress on lipid metabolism helps to explain the theory. This study established a raged-rat model (refers to a method described in Experiment of Zoology, issued by Science Press, China) by clipping its tail, three times a day for 30 minutes each time. In the preliminary experiments, we found that the method mentioned in Experiment of Zoology caused huge damages to rats, we determined to adjust this tail clipping to three times a day. We found acute stress affected the behaviors of rats, which is proved in the open-field test, a method used to evaluate the behavior of animal. The horizontal scores of open-field test reflects the excitability of rats, while vertical scores reflects the rat's to the environment [21,22]. Both the activities of the model group are lower than the normal group, the result shows excitability and exploration activities of rats decreased due to being subjected to stress.
With normal rats, we found serum ALB level was lower in the model group, with higher GLB, LDL-C and lower HDL-C, demonstrated acute stress may damage rat's liver, leading to a reduction in ALB production, an increase in GLB. ALB is manufactured in the liver, when liver is impaired, it decreases the production of ALB. At the same time, GLB is manufactured by immune organ, when there is an 'enemy' in the body, GLB increase in production. Serum concentrations of HDL-C have strong inverse correlations with the risk of cardiovascular disease, independently of LDL-C levels, indicate that acute stress may influencing the HDL-C and LDL-C levels of serum, thus affecting the liver lipid metabolism. Due to the stress of time is not long or strong enough, the extent of the damage of liver cells is not particularly serious. So there are no significant differences in ALT and AST levels between the normal group and model group in the present study.
In this study, the liver tissue of model group exhibited a lower gene expression of ABCG5, ABCG8 and a higher SR-BI level, when compared to normal rats. Furthermore, liver protein expression of ABCG5 and ABCG8 were lower in the model group than the normal group, while SR-BI was higher in the model group than the normal group. ABCG5 and ABCG8 are ATP-binding cassette transporters, which expressed mainly in the liver and small intestine, mediated lipid membrane unidirectional outflow into the extracellular. After acute stress applied, ABCG5 and ABCG8 decreased expression in the liver, henced the intracellular lipid efflux was suppressed. The results show that, acute stress may affect the liver lipid efflux from the cells, thereby affecting the normal operation of the lipid metabolism, and may also be one of the reasons the Chinese say 'raged impairing liver'. SR-BI is one kind of HDL receptors, first determined at the molecular level, having highly binding force with HDL, and mediated hepatic selective uptake of cholesterol ester. Increased the expression of liver SR-BIcan be effective in promoting reverse cholesterol transport. But in this study, SR-BI expression increased after acute stress was applied. This may be in the early acute stress process, mature HDL decreased in production, SR-BI expression in the liver cell membrane transient up-regulation in order to better capture of HDL. Specific mechanism needs to be followed up in future studies.

Conclusions
In conclusion, we confirmed here for the first time acute stress decreased the expression of hepatic ABCG5 and ABCG8 besides up-regulated SR-BIin raged rats. Our data shown that acute stress harms normal liver functions, affects the level of lipid metabolism. Elevated serum GLB and LDL-C levels, reduced serum ALB and HDL-C levels, inhibited of reverse cholesterol transporter gene expression may all contribute to the harmful effects of acute stress. Our findings help to explain the Chinese medicine terminology of raged impairing liver.