Tocotrienols inhibit lipopolysaccharide-induced pro-inflammatory cytokines in macrophages of female mice

Background Inflammation has been implicated in cardiovascular disease, and the important role of proteasomes in the development of inflammation and other macrophage functions has been demonstrated. Tocotrienols are potent hypocholesterolemic agents that inhibit β-hydroxy-β-methylglutaryl coenzyme A reductase activity, which is degraded via the ubiquitin-proteasome pathway. Our objective was to evaluate the effect of tocotrienols in reducing inflammation. Lipopolysaccharide (LPS) was used as a prototype for inflammation in murine RAW 264.7 cells and BALB/c female mice. Results The present results clearly demonstrate that α-, γ-, or δ-tocotrienol treatments inhibit the chymotrypsin-like activity of 20 S rabbit muscle proteasomes (> 50%; P < 0.05). Chymotrypsin, trypsin, and post-glutamase activities were decreased > 40% (P < 0.05) with low concentrations (< 80 μM), and then increased gradually with concentrations of (80 - 640 μM) in RAW 264.7 whole cells. Tocotrienols showed 9 - 33% (P < 0.05) inhibitions in TNF-α secretion in LPS-stimulated RAW 264.7 cells. Results of experiments carried out in BALB/c mice demonstrated that serum levels of TNF-α after LPS treatment were also reduced (20 - 48%; P < 0.05) by tocotrienols with doses of 1 and 10 μg/kg, and a corresponding rise in serum levels of corticosterone (19 - 41%; P < 0.05) and adrenocorticotropic hormone (81 - 145%; P < 0.02) was observed at higher concentrations (40 μM). Maximal inhibition of LPS-induced TNF-α was obtained with δ-tocotrienol (10 μg/kg). Low concentrations of δ-Tocotrienols (< 20 μM) blocked LPS-induced gene expression of TNF-α, IL-1β, IL-6 and iNOS (> 40%), while higher concentrations (40 μM) increased gene expression of the latter in peritoneal macrophages (prepared from BALB/c mice) as compared to control group. Conclusions These results represent a novel approach by using natural products, such as tocotrienols as proteasome modulators, which may lead to the development of new dietary supplements of tocotrienols for cardiovascular diseases, as well as others that are based on inflammation.


Background
Lipopolysaccharide (LPS), which is expressed on the outer membrane of essentially all Gram-negative bacteria, is a potent inducer of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α interleukin-1β (IL-1β), IL-6, IL-8, arachidonic acid metabolites and nitric oxide [1]. LPS can also induce corticosteroid production by the host, which tends to suppress further production of pro-inflammatory cytokines. Some conditions leading to dysregulated production of inflammatory cytokines by the host can produce profound alterations in metabolic, cardiovascular, immunological, haemostatic, and endocrine functions, which may ultimately lead to septic shock [1][2][3]. Less profound inflammatory responses have also been implicated in the pathogenesis of atherosclerosis, cancer, stroke and diabetes in human subjects [4][5][6][7].
Proteasomes are essential for numerous physiological processes, including signal transduction, transcriptional activation, cell cycle progression, and certain immune cell functions [8]. We have reported a potentially important central role for proteasomes in inflammation and other macrophage functions [8]. Proteasomes often exist as 26 S multi-subunit complexes containing a 20 S proteolytic proteasome and a 19 S regulatory complex. Correspondingly, the 20 S proteasome is comprised of a variety of distinct protein subunits that account for the different proteolytic activities of the 20 S proteasome. Several different exogenous inhibitors or activators of proteasome function have been described, and these inhibitors act by blocking, or activating, the proteolytic activity of the individual protein subunits of the 20 S proteasome.
Moreover, the far superior efficacy of tocotrienols versus tocopherols (vitamin E) as antioxidants has been established, and δ-tocotrienol is found to be the most potent among the known tocotrienols [10,17,18,22]. Tocotrienols also show non-antioxidant properties in various in vitro and in vivo models. Perhaps most importantly, tocotrienols interact with the mevalonate pathway leading to the lowering of cholesterol levels, the prevention of cell adhesion to endothelial cells, the suppression of tumor cell growth, and glutamateinduced neurotoxicity [10][11][12]23,24].
The present investigation was carried out to evaluate the mechanisms by which tocotrienols inhibit inflammation. Murine systems were chosen in the present study, because they are relatively resistant to the lethal effects of endotoxin compared to rabbit and sheep [1,3,25]. Recently, rodent models have been developed which are very sensitive to bacterial endotoxin and thus, female mice were chosen due to their increased sensitivity to LPS-induced inflammation. These experiments were designed to test our hypothesis that tocotrienol treatment modulates proteasomal activity, induces corticosteroids synthesis, and blocks LPS-induced signaling pathways that contribute to the inflammatory process.
Effects of various tocols (tocopherols + tocotrienols) on the chymotrypsin-like activity of 20 S rabbit muscle proteasomes Proteasomal activities of the 20 S rabbit muscle proteasomes (0.4 μg/mL) were assayed with synthetic peptide substrates in 0.02 M Tris-HCl buffer (pH 7.2). The substrate used for the chymotrypsin-like activity was 100 μM of succinyl-Leu-Leu-Val-Tyr-amino-methyl-coumarin. Fluorescence was measured (absorption at 360 nm and emission at 460 nm) using an FLX 800 microplate fluorescence reader (Bio-Tek Instruments, Winooski, VT).

Cell culture and maintenance
The RAW 264.7 cells or mouse peritoneal macrophages were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum (FBS) and 10 mg gentamicin (in 500 mL) at 37°C in a humidified atmosphere with 5% CO 2 as described previously [27]. Cells were cultured in 6-well plates as described in the legends to the figures.
Effects of δ-tocotrienol (concentrations of 10 -640 μM) after 60 min treatment on different proteasomal activities (chymotrypsin-like, trypsin-like, and postglutamase) in RAW 264.7 whole cells In order to check the comparative inhibitory effect of tocols, only the most potent δ-tocotrienol was tested on the chymotrypsin-like, trypsin-like, and post-glutamase activities of the proteasome using RAW 264.7 whole cells; the following experiment was carried out. RAW 264.7 cells (10 × 10 4 cells/100 μl/well) were added in white plates 96-well, Fisher, 0877126), followed by the addition of various concentrations of δ-tocotrienol (10, 20, 40, 80, 160, 320, or 640 μM in 100 μL; dissolved in 0.2% dimethyl sulfoxide (DMSO). The mixtures were incubated at 37°C in an incubator at 5% CO 2 for 60 min. After incubation period, the cells in the 96-well plates were taken out 20 min prior to the addition of Caspase-Glo reagent (brought to room temperature before addition to the wells). Caspase-Glo reagent (100 μL) was added to each well to a total volume of 200 μL/well (tris buffer, pH 7.5; 0.02 M). The plate were covered with a plate sealer, removed from light and incubated at room temp for 10 min. The relative luminescence units (RLU) of assays were read with a Promega Plate Luminometer.
The chymotrypsin-like, trypsin-like, or post-glutamase activities were quantitated by measuring luminescence after stimulation of RAW 264.7 whole cells with various doses of δ-tocotrienol in a Luminometer (Promega), according to the directions of manufacturer.
Effects of a-, g-, and δ-tocotrienols on release of LPSinduced TNF-a in serum of 6-wk-old female BALB/c mice All mice used in this study received humane care in compliance with the principles of laboratory animal care formulated by the National Society of Health Guide for the Care and Use of Laboratory Animals (DHHS Publication No. [NIH] 85 -23, revised 1985). The experimental procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of UMKC, Medical School, MO. Female BALB/c mice were acclimatized to the new environment for fourteen days and were fedad libitum regular commercial mice diet and had free access to water throughout the experiment. A 12 h light and 12 h dark cycle was maintained during feeding period. All samples were dissolved in 5% triethylamine solution.
Effects of a-, g-, and δ-tocotrienols on the induction of corticosterone and adrenocorticotropic hormone (ACTH) in serum of LPS-stimulated 6-wk-old female BALB/c mice To further probe the anti-inflammatory effects of various tocotrienols in mice, we queried whether tocotrienols would also induce the serum levels of corticosterone and adrenocorticotropic hormone (ACTH) after LPS treatment of mice as reported recently [3]. The levels of serum (used from the above experiment) corticosterone and ACTH were estimated according to published method [1,3].
Isolation of thioglycollate-elicited peritoneal macrophages from BALB/c mice, total cellular RNA isolation and RT-PCR Ten 4-wk-old female BALB/c mice were acclimatized to the new environment for fourteen days, and thioglycollate-elicited peritoneal macrophages were prepared as described previously [29]. Macrophages (2 × 10 5 ) were treated with either pure LPS (10 ng/treatment), LPS + α-tocopherol (25, 50, or 100 μM), LPS + δ-tocotrienol (10, 20, or 40 μM). All the samples were dissolved in 0.2% dimethyl sulfoxide (DMSO). The assay mixtures were incubated at room temperature for 4 h and then centrifuged at 2,000 rpm for 20 min. The supernatants were removed and concentrations of TNF-α were determined using Quantikine M ELISA kit (R&D System, Minneapolis, MN) according to the manufacturer's directions [29]. The total RNA was isolated from each pellet with RNeasy mini kit according to the manufacturer's instructions. To check the purity of the total RNA, Reverse transcriptase polymerase chain reaction (RT-PCR) was conducted using a 1-step kit (Qiagen, Chatsworth, CA) according to the manufacturer's instructions.

Statistical analyses
The analyses demonstrated the affect of various isomers of tocols (α-tocopherol or α-, γ-, δ-tocotrienols) within the groups. Stat View software (version 4.01, Abacus Concepts, Berkeley, CA) was used for the analyses of treatment-mediated effects as compared to the control group. Treatment-mediated differences in various inflammatory markers variables were evaluated using a two-way ANOVA, and when F test indicated a significant effect, the differences between the means were analyzed by a Fisher's protected least significant and least difference test. Data were reported as means ± SD in text and Tables. The level for statistical significance level was established at 5% (P < 0.05).

Results
The results of the present study on the inhibition of inflammation by tocotrienols are presented in the same in two different formats. For each figure, 'A' shows the raw values for each of the treatments and controls, and 'B' shows the percent change compared to controls.
Inhibition of chymotrypsin-like activity of 20 S rabbit muscle proteasomes by various tocols (tocopherols + tocotrienols) We have previously shown that the proteasome is a central regulator of inflammation; we therefore queried whether tocotrienols also affect proteasome activity, thus affecting the induction of cytokines. The results of this study reveals that inhibition of chymotrypsin-like activity of the 20 S rabbit muscle proteasomes was dosedependent between 4 μM and 16 μM for α-tocotrienol (6% -36%), γ-tocotrienol (12% -32%), and δ-tocotrienol (51% -55%) as compared to control groups (Figure 2A  &2B). An insignificant reduction (5%) in 20 S proteasomes activity was observed with α-tocopherol (Figure 2A &2B). These studies further suggest that δ-tocotrienol is the most effective isomer of the tocotrienols for inhibiting the chymotrypsin-like activity of rabbit muscle proteasomes.
Impact of δ-tocotrienol (concentrations of 10 -640 μM) on different proteasomal active sites (chymotrypsin-like, trypsin-like, and post-glutamase) in RAW 264.7 whole cells after 60 min treatment The effects of δ-tocotrienol on the chymotrypsin-like, trypsin-like, and post-glutamase activities of proteasomes were also carried out in RAW 264.7 murine macrophages. The chymotrypsin-like activities decreased to 46% of controls with 40 μM δ-tocotrienol treatment of cells and then increased gradually as shown in Figure 3. A similar trend was observed with the trypsinlike activity and post-glutamase activities in response to δ-tocotrienol (Figure 3).
Inhibition of the secretion of TNF-a in LPS-stimulated RAW 264.7 cells by various tocols (a-tocopherol, a-tocotrienol, g-tocotrienol or δ-tocotrienol) The effects of tocotrienols on the secretion of TNF-α with, and without, LPS-stimulation in RAW 264.7 cells was investigated. A significant reduction of secretion of TNF-α, 9% -33% (P < 0.05) was observed with tocotrienols in LPS-stimulated RAW 264.7 cells in a dosedependent manner (Figure 4A &4B). Tocotrienols did not inhibit TNF-α secretion in the absence of LPS stimulation (data not shown in the figure). Cell viability for all above experiments was 90% -95%. To further probe the anti-inflammatory effects of various tocols in mice, we queried whether dietary supplementation with tocotrienols would also block LPSinduced TNF-α secretion in mice. A dose-dependent inhibition (20% to 48%; P < 0.05) of LPS-induced serum TNF-α levels was observed with α-, γ-, and δ-tocotrienols as compared to control diets (Figure 5A &5B). The reduction with α-tocopherol (5% -9%) was insignificant as compared to control or tocotrienol treatments ( Figure  5A &5B). These results also suggest that δ-tocotrienol is the most effective isomer in reducing LPS-induced TNF-α secretion in mice.
a-, g-, and δ-Tocotrienols induce corticosterone in serum of 6-wk-old BALB/c mice We have previously shown that LPS treatment of mice induces high endogenous corticosterone levels, and that corticosterone levels are inversely correlated with TNF-α serum concentrations [9]. Therefore, to further explore mechanisms responsible for the anti-inflammatory effects of tocotrienols, we queried whether tocols increase corticosterone levels produced in response to LPS. For this experiment BALB/c mice were injected i.p. with tocotrienols. A significant (P < 0.02) rise in LPSinduced serum corticosterone (19%, 31%, and 41%) levels was induced by treatment with α-, γ-, or δ-tocotrienols as compared to the control group ( Figure 6A  &6B). These results suggest that the decrease in the synthesis of TNF-α could be due to the rise in the endogenous corticosteroids, which may modulate the synthesis of inflammatory cytokines [3]. Concentrations of -tocotrienol in μM.

Chymotrypsin-like
Trypsin-like Post-glutamase Qureshi et al. Lipids in Health and Disease 2010, 9:143 http://www.lipidworld.com/content/9/1/143 described above. A corresponding significant (P < 0.02) dose-dependent rise in LPS-induced serum adrenocorticotropic hormone levels was observed (81%, 118%, and 145%) by treatment with α-, γ-, or δ-tocotrienols, respectively, as compared with the control group ( Figure 7A  &7B). An insignificant rise in the levels of corticosterone (7%) and adrenocorticotropic hormone (14%) with α-tocopherol was also observed (Figure 6B &7B). In summary, these results clearly indicate that δ-tocotrienol is most effective in inhibiting the serum levels of TNF-α and also in inducing the serum levels of corticosterone and adrenocorticotropic hormone as compared to α-, γ-tocotrienols and α-tocopherol, which were the least effective compounds as compared to their respective control groups.
δ-Tocotrienol inhibits LPS-induced TNF-a, IL-1b, IL-6, and iNOS gene expression in thioglycollate-elicited peritoneal macrophages of 6-wk-old BALB/c mice In order to define the mechanism of action of tocotrienols with respect to lowering inflammation, thioglycollate-elicited peritoneal macrophages were prepared from BALB/c female mice, and treated with α-tocopherol, or δ-tocotrienol 1 h before, or at the same time as LPS. After 4 h of the treatment, total cellular RNA was extracted and reversetranscribed, and gene analysis was performed by RT-PCR and southern blot analyses. The results of gene expression studies clearly demonstrate that δ-tocotrienol is most effective in blocking LPS-induced gene expression of TNF-α Figure 8A &8B, IL-1β, IL-6 and iNOS at low doses of 10 μM and 20 μM (Figure 9A &9B). There were significant (P < 0.02) reductions of TNF-α (45%), IL-1β (63%), IL-6 (46%) and iNOS (32%) mRNA with a dose of 20 μM compared to their respective control values, and significant increases (40%, 17%, 26%, 13%) in the levels of these mRNA's, with a dose of 40 μM of δ-tocotrienol, as compared to their respective 20 μM control values (Figure 8B &9B). These results are in agreement with earlier results of tocotrienols on the activities of chymotrypsin-like, trypsin-like and postglutamase in RAW 264.7 whole cells (Figure 3). The α-tocopherol (doses of 25, 50 or 100 μM) did not have any impact on the gene expression of TNF-α, IL-1β, IL-6 and iNOS compared to their respective control values ( Figure 8A, Figure 9B).

Discussion
It is well established that LPS-induced TNF-α production requires activation of NF-B and activation of proteasomal activity. The results presented here demonstrate that tocotrienols block production of key pro-inflammatory cytokines induced by LPS. We hypothesized that this blockade of LPS-induced inflammation by tocotrienols could be due to modulation of proteasomal activity [31], since tocotrienols have been shown to be effective inhibitors of β-hydroxy-β-methylglutaryl coenzyme A (HMG-CoA) reductase, (the rate-limiting enzyme in cholesterol biosynthesis), and are very potent antioxidant and hypocholesterolemic agents [12]. It has also been demonstrated that HMG-CoA reductase is degraded via the ubiquitin-proteasome pathway [32,33]. We have shown that tocotrienols can inhibit chymotrypsin-like activity of 20 S rabbit muscle proteasome, and that δ-tocotrienol specifically inhibits the chymotrypsin-like, trypsin-like and post-glutamase activities of the proteasome in a dose-dependent manner in RAW 264.7 whole cells at concentrations below 80 μM; at higher concentrations (80 -640 μM) tocotrienole enhance these activities as has been previously reported for lactacystin [34,35].
Tocotrienols also inhibit the LPS-stimulated secretion of TNF-α in RAW 264.7 cells and in serum of BALB/c mice, and a corresponding significant (P < 0.02) rise was observed in the serum levels of corticosterone and adrenocorticotropic hormone compared to controls. Moreover, δ-Tocotrienol is effective in reducing LPS-induced gene expression of TNF-α, IL-1β IL-6 and iNOS at low concentrations. Interestingly, at high concentrations δ-Tocotrienol activates chymotrypsin-like, trypsin-like, and post-glutamase activities, and upregulates LPSinduced gene expression of TNF-α, IL-1β, IL-6 and iNOS. It seems that tocotrienols actually upregulates the proteasomal activity, and thus can upregulate LPSstimulated transcription of several pro-inflammatory genes at higher concentrations. Therefore, the capacity of δ-tocotrienol to modulate inflammation may be attributable, in part, to inhibition and activation of the chymotrypsin-like, trypsin-like and post-glutamase' activities of mouse macrophages.
The present results also demonstrated that δ-tocotrienol is the most effective tocol among the known, αand γ-tocotrienols for inhibition or induction of various cytokines involved in inflammation, whereas α-tocopherol does not play any role in inhibiting LPS-induced inflammation. This conclusion has been further confirmed in a recent study [32] which demonstrated that δ-tocotrienol is very effective in inducing ubiquitination and degradation of HMG-CoA reductase. δ-tocotrienol also blocks the processing of sterol regulatory element-binding proteins-2 (SREBPs-2) which, when activated, can enhance transcription of genes encoding cholesterol biosynthetic enzymes, including HMG-CoA reductase [32]. On the other hand, tocopherols neither increase degradation of reductase, nor decrease SREBP-2 processing [32].
The unsaturated isoprenoid side-chain in tocotrienol molecules is important for its biological activity and it was suggested that this could stimulate binding of HMG-CoA reductase to Insig (endoplasmic reticulum membrane protein). Alternatively, the side-chain might allow improved penetration and distribution in cell membranes [32,33]. This may contribute to the differences observed in the potency of tocotrienols versus tocopherols in stimulating HMG-CoA reductase in vitro ubiquitination [32,33] thus confirming our previous and present results showing that δ-tocotrienol (one methyl group) is more potent than γ-tocotrienol (two methyl groups) due to the number of methyl groups which abolishes regulatory activity with respect to HMG-CoA reductase degradation and SREBP-2 processing [10,11,32].  Effects of a-tocopherol, a-tocotrienol, g-tocotrienol and δ-tocotrienol on the induction of adrenocorticotropic hormone in serum of LPS-stimulated 6-wk-old BALB/c female mice. Three mice for each concentration were injected i. p. with various tocols in 5% triethylamine solution (0.2 mL/mouse) one hr prior to LPS challenge, after conditioning them for 14 days. Mice were bled after 2 h later to collect serum. Serum adrenocorticotropic hormone levels were determined according to published procedure (3). Values in a column with a different superscript letters are significantly different at P < 0.05, A = values; B = percentages.  IL-1b IL-6 iNOS Figure 9 Effects of a-tocopherol and δ-tocotrienol on the gene expression of IL-1b, IL-6, and iNOS in LPS-stimulated peritoneal macrophages of 6-week-old BALB/c female mice. The RT-PCR procedure is described in the experimental section. The ratios of relative optical density (cytokines/β-actin) of each treatment for each marker was used to draw this figure, A = values; B = percentages.