Effect of Launaea procumbens on thyroid glands lipid peroxidation and hormonal dysfunction: a randomized control trial

Background Launaea procumbens (Roxb.) Amin is traditionally used in Pakistan for the treatment of hormonal disorders and oxidative stress. The present study was aimed to evaluate the efficacy of Launaea procumbens methanol extract (LPME) against KBrO3-induced oxidative stress and hormonal dysfunction in thyroid. Methods To examine the effects of LPME against the oxidative stress of KBrO3 in thyroid tissue, 36 male albino rats were used. Protective effects of LPME were observed on thyroid hormonal levels, activities of antioxidant enzymes, lipid peroxidation (TBARS) and DNA damage. Results Treatment with KBrO3 significantly (P < 0.01) reduced the levels of T3 (55.13 ± 1.93) and T4 (14.7 ± 1.78) and increased TSH (55.13 ± 1.93) levels. KBrO3 exposure in rats reduced the activities of antioxidant enzymes viz.; CAT (1.16 ± 0.08); SOD (12.0 ± 0.08), GST (17.7 ± 1.1) and GSR (54.3 ± 2.1) but increased lipid peroxidation (20.3 ± 0.71) and DNA (30.4 ± 2.0) damage. Co-administration of LPME significantly (P < 0.01) improved these alterations with respect to hormonal levels, activities of antioxidant enzymes and lipid peroxidation close to those seen in control rats. Conclusion These results suggest that LPME can protect thyroid tissue against oxidative damage, possibly through the antioxidant effects of its bioactive compounds.


Background
Potassium bromate (KBrO 3) molecular weight 166 g/mol is an oxidizing agent. KBrO 3 has been used is in industries for the formation of hair solution and cosmetics. Potassium bromate is formed as by product during ozonization of water, causes infections and has been classified as 2B group toxic chemical a probable human carcinogen [9]. KBrO 3 causes renal cell and thyroid carcinomas in rats, hamsters and mice when exposed chronically [15]. It has been investigated that potassium bromate produces free oxygen radicals which causes oxidative stress and DNA damages [20]. KBrO 3 causes nephrotoxicity and hepatotoxicity; decreases the tissue soluble proteins, antioxidant enzymes. The decrease of antioxidant enzymes are due to reactive oxygen species (ROS) produced by metabolism of potassium bromate.
Potassium bromate depleted GSH content in various tissues which causes decrease in phase II metabolizing enzymes like GSH-Px and glutathione reductase (GSR). It also increases TBARS contents, causes lipid peroxidation and disrupts liver profile including γ-GT, ALP and protein concentration [4]. Medicinal plants and nutraceuticals play important role in improving human health and phytomedicine [24]. These medicinal plants are composed of some bioactive phytochemical substances, regulates various physiological and molecular action in living organisms. Today many natural products extracted from medicinal plants are being tested for the presence of new drugs with new modes of pharmacological action. Special features of higher plants are their capacity to produce a large number of secondary metabolites [2].
Launaea procumbens (Roxb.)Amin. (Asteraceae) is an annual herb having simple leaves and yellow flowers found in waste places, vacant lots and in cultivated fields throughout Pakistan. Launaea procumbens was used as a food and washing agent [27] rheumatism, galactogogues, and increases milk production. Eye redness and itchiness are treated with Launaeaprocumbens and also traditionally used in kidney (painful urination), liver and sexual diseases like gonorrhoea [23]. The antimicrobial activity of ethanolic and aqueous fractions of Launaeaprocumbens was checked against various bacterial and fungal pathogens and reported that proved that ethanolic extract show maximum activity while theaqueous extract was inactive. Launaea procumbensis generly classified as, Kingdom (Plantae), Order (Asterales), Family (Asteraceae), Tribe (Cichorieae), Genus (Launaea), and Species (procumbens). Chemical characterization showed that Launaeaprocumbens composed of salicylic acid, vanllic acid, synergic acid, 2-methyl-resercinol, gallic acid and used against plant pathogenic fungi, nematocides and as allelopathic for inhibition of plant growth [26].
In the present research work we arranged to investigate the protective effects of plant extracts against KBrO 3 induced thyroid dysfunctions in rats.

Plant collection and extraction
Launaea procumbens whole plant was collected from District Bannu during summer season 2016. After shad drying of plant at room temperature (25°C), it was ground mechanically to get fine powder. Among powder, 1 kg of fine powder was socked in 70% methanol (1.5 L) and shacked randomly. After a week the extract was filtered viaWhatman filter paper No.1. Filtrate was concentrated using rotary evaporator at 38°C to get methanol crude extractand was kept at 4°C in the refrigerator for further in vitro investigation.

Experimental procedure
Sprague-Dawley male rats were divided into sixteen groups (06 rats). Group 1 was control while Group II received DMSO and saline (0.20% aqueous NaCl solution) orally (Wednesday and Saturday) at a dose of 3 ml/kg b.w. Group III was administered high purity grade KBrO 3 (99.9%) 20 mg/kg b.w in 0.20% aqueous NaCl solution (Monday and Thursday) intragastrictly. Group IV and V were given 100; 200 mg/kg b.w of crude methanol extract (Wednesday and Saturday) of L. procumbens (LPME) after 48 h of KBrO 3 treatment. However, Group VI was given only (LPME) at a dose of 200 mg/kg b.w (Wednesday and Saturday). All these treatments were given twice a week for 4 weeks. After completion of experiment all the animals were kept on normal feed without any treatment for at least 24 h before the dissection of animals. Animals were given chloroform anesthesia after urine collection and dissected from ventral side. Blood was collected in the falcon tube and centrifuged for serum and stored in refrigerator. Thyroid glandwas removed and washed in ice cold saline, dried with blotting paper and weighted. After weighing the tissues were divided into two portions. One part was cut off and stored in fixative sera for histology while the other portion was treated with liquid N 2 and stored at −70°C for further biochemical and molecular studies.

Genotoxicity assays
DNA damages were assessed using protocol of Wu et al. [28] qualitatively as well as quantitatively.

Histopathalogical studies
Cellular changes were observed light microscope at 40×.

Statistical analysis
To determine the treatment effects one way analysis of variance was carried by computer software SPSS 13.0. Level of significance among the various treatments was determined by LSD at 0.05% level of probability.

Serum Biochemistry
Effects of methanol fraction on the serum level of thyroid hormones like TSH, T 3 and T 4 are shown in Table 1. Treatment of KBrO 3 caused reduction in the secretion of T 3 , T 4 and increased the secretion of TSH comparatively to non treated rats. Co-administration of LPME significantly decreased the hormonal secretion in a dose dependent way.

Assessment of antioxidant profile
Antioxidant enzymes play a crucial role in detoxification of free radicals. KBrO 3 induction in rats significantly (p > 0.01) decreased the amount of tissue soluble protein and activities of first level antioxidant enzymes viz.; CAT, POD and SOD as compare to control. Postadministration of 100 and 200 mg/kg b.w., LPME markedly (p > 0.05) reduced the intoxication and reversed the changes to normal level ( Table 2). Table 3 revealed the activities of phase II metabolizing enzymes viz.; GST, GSH-Px, GSR, QR, and γ-GT in various groups. KBrO 3 -treatment in rats significantly (p < 0.01) depleted the activity of GST, GSH-Px, GSR and QR, whereas increased the activity of γ-GT comparatively to control group. The modulation were significantly (p > 0.01) reversed by the co-treatment of 100 and 200 mg/kg b.w., LPME near to control group.
TBARS, H 2 O 2 , GSH and tissue nitrite are key marker of lipid peroxidation and cellular oxidative stress. KBrO 3 effects on the content of TBARS, H2O2, and GSH are shown in Table 4. Administration of 20 mg/kg b.w., of KBrO 3 significantly (p < 0.01) reduced the concentrations of GSH while increased H 2 O 2 and TBARS as compare to control. TBARS, tissue nitrite was significantly reduced (p < 0.01) by LPME decreased the level of TBARS at 100 and 200 mg/kg (p < 0.01) as compared to KBrO 3 group. Level of GSH was improved while H 2 O 2 was reduced (p < 0.01) by LPME at 100 mg/kg and 200 mg/kg b.w, respectively.

Thyroid weight and genotoxicity
Effects of LPME against KBrO 3 administration on rats thyroid weight, relative tissue weight and % DNA fragmentation are shown in Table 5. KBrO 3 caused a significant (p < 0.01) increased in thyroid tissue  Indicate significance from the control group at P < 0.01 probability level ++ Indicate significance from the KBrO 3 group at P < 0.01 probability level weight and relative thyroid tissue weight and %DNA damages as compared to non treated control group. Post-treatment of LPME erased the toxic effect of KBrO 3 on rat and reduced the thyroid weight, relative tissue weight and % DNA fragmentation and significantly (p < 0.01) reversed towards control group.

Histopathology of thyroid tissue
Microscopic examinations of various treated groups are shown in Table 6. Administration of KBrO 3 caused colloid depletion and cellular hypertrophy, blood vessels congestion, inflammatory cells infiltrations and follicular shape disruptionand follicular cells hyperplasia. Posttreatment with LPME significantly erased the injuries near to control rats.

Discussion
Thyroid hormones are essential for normal growth of organs, development, function and also regulate hepatocytes metabolism while liver in turn metabolizes thyroid hormones. It means that liver and thyroid hormones are connected with one another, dysfunction of one causes disturbance of other [17]. The hypothalamus releases thyrotrophic-releasing hormone (TRH), which stimulates pituitary gland to release thyroid stimulating hormone (TSH), which in turn promotes thyroid cells to produce thyroid hormones. When level of thyroid hormone is low than TSH and TRH are high, try to increase thyroid hormone and causes risk of thyroid tumor in rats [5]. Our results showed that administration of KBrO 3 depleted the secretion of thyroid hormones i.e., T 3 and T 4 and elevated TSH level in serum of rats which was recovered by LPME. Similar results were reported by Hamidian et al., [7]. The present study revealed that marked changes were induced by KBrO 3 in absolute b.w and % increase in body weight. KBrO 3 treatment of rats significantly decreased the b.w, as compare to the non-treated control group. Our results are similar to the findings reported by Farombi et al. [4] that KBrO 3 treatment to rat decreases the b.w non significantly as compare to control group. Other study also determined that that low amount of KBrO 3 is not toxic as well as mutagenic (Yamaguchi et al., [29]. Increased lipid peroxidation and/or altered non-enzymatic and enzymatic antioxidant systems contributed oxidative stress. Various reports revealed that various enzymatic and non-enzymatic systems in mammalian cell have the capability to reduced ROS and other free radicals [25]. Toxicity induced with KBrO 3 results from its metabolism through CYP 450 into free radicals that in turns leads to oxidative damages. In thyroid presence of cytochrome P450 enzyme system has not been established and injuries induced in thyroid are considered indirectly via the production of metabolites in other organs. In the present study induction of KBrO3 significantly alter the enzymatic level which was reversed by co-treatment of LPME. Similar reports were obtained by the exposure of KBrO 3 in rats which has been restored by  Indicate significance from the control group at P < 0.01 probability level ++ Indicate significance from the KBrO 3 group at P < 0.01 probability level supplementation of Ficus racemosa and a natural antioxidant kolaviron [14]. Free radicals react with DNA to form a mutagenic pirimedopurinone adduct (M1G) of deoxyguanosine [18] causes genomic instability and damages. The data revealed that the treatment of KBrO 3 causes significant oxidative DNA damage in thyroid tissue of rats which are reversed by LPME. Similar investigation was reported by Khan et al., [13] during study of protective effects of Digeramuricata. These results show that the extract contains bioactive phenolic and poly phenolic compounds which play important role in DNA repair. Data of the present study revealed that KBrO 3 treatment in rats caused colloids depletion and hypertrophy, blood vessels congestion, follicular shape degradation and hyperplasia in thyroid. Treatment with LPME significantly erased the injuries. Similar observation was reported by Hooth et al. [8] during drinking water exposure of sodium chlorate in rats.