Associations between GUCY1A3 genetic polymorphisms and large artery atherosclerotic stroke risk in Chinese Han population: a case-control study

Background Previous genome-wide association studies have found two single nucleotide polymorphisms (SNP) rs7692387 and rs1842896 located on or near the GUCY1A3 gene were associated with coronary artery disease (CAD). GUCY1A3 was considered to be involved in the process of atherosclerosis, but there was little information about the association between genotypic polymorphisms of the GUCY1A3 and large artery atherosclerotic (LAA) stroke. This study aimed to investigate the associations between the GUCY1A3 rs7692387, rs1842896 polymorphisms and LAA stroke susceptibility. Methods A total of 298 LAA stroke patients and 300 control subjects from a southern Chinese Han population were included. SNaPshot technique was used for genotype analysis. Associations between genotypes and LAA stroke susceptibility were analyzed with logistic regression model. Results Our study found that under the recessive model (TT vs. GT + GG), the GUCY1A3 rs1842896 polymorphism was significantly correlated with LAA stroke (OR = 1.48, 95%CI: 1.07–2.04, P = 0.018). After adjustment for its effects on age, gender, cigarette smoking, total cholesterol, low-density lipoprotein cholesterol, HbA1c, hypertension, diabetes mellitus, and CAD, the rs1842896 TT genotype retained association with increased susceptibility to LAA stroke (recessive model: adjusted OR = 1.96, 95%CI: 1.22–3.17, P = 0.006). However, association between rs7692387 polymorphism with LAA stroke was not observed. Conclusion Our results indicate that the GUCY1A3 rs1842896 polymorphism is an LAA stroke risk factor in Southern Han Chinese.


Background
Ischemic stroke (IS) is a disease with high morbidity and mortality, which affects people across the world and causes heavy economic burden for families and the whole society [1]. According to the TOAST (Trial of Org 10,172 in Acute Stroke Treatment) classification [2], IS can be divided into five etiologic subtypes, including large artery atherosclerotic (LAA) stroke. LAA stroke carries the highest risk of early neurological deterioration and early recurrent stroke in comparison to other IS subtypes [3,4]. LAA stroke is attributed to atherosclerosis, where the development of thrombosis may cause occlusion of cerebral artery, and lead to irreversible brain damage. Atherosclerosis is a typical complex disease process caused by multiple environmental and genetic factors and their interactions [5] . However, the causative genes are largely unknown. To date, genomewide association studies (GWAS) have identified several genomic loci are associated with atherosclerosis-related diseases, such as LAA stroke and coronary artery disease (CAD). Some of these variants on chromosome 4q32.1 tags the GUCY1A3 gene (according to a new nomenclature also known as GUCY1A1) [6,7].
The GUCY1A3 gene encodes the α1 subunit of the soluble guanylyl cyclase (sGC). The sGC complex, a heterodimeric enzyme comprising a β1 subunit and either an α1 or α2 subunit, is the only known receptor for nitric oxide (NO) and catalyzes the formation of the second messenger cyclic guanosine 3′,5′-monophosphate (cGMP). cGMP regulates a vast array of physiological processes, including neurotransmission [8], vascular smooth muscle cells (VSMCs) relaxation [9], and inhibition of platelet aggregation [10]. Previous GWAS have found rs7692387 [11] and rs1842896 [6] single nucleotide polymorphisms (SNPs) located on or near the GUCY1A3 gene were associated with coronary artery disease (CAD). Although the nidi of CAD and LAA stroke are different, they share the same pathophysiological basis: atherosclerosis.
GUCY1A3 was considered to be involved in the process of atherosclerosis, but there was little information about the association between genotypic polymorphisms of the GUCY1A3 and large artery atherosclerotic (LAA) stroke. To our knowledge, the genetic evidence on the associations between the GUCY1A3 rs7692387, rs1842896 polymorphisms and susceptibility of LAA stroke has not been reported previously. Therefore, the present study was conducted to evaluate the associations between the two SNPs and LAA stroke susceptibility in a Southern Chinese Han population.

Study population
A total of consecutive unrelated 298 LAA stroke patients were enrolled from September 2016 to December 2017 in this study. IS was defined according to the World Health Organization definition as "rapidly developing clinical signs of focal (or global) disturbance of cerebral function lasting more than 24 hours with no apparent cause other than of vascular origin", with evidence of cerebral infarction in clinically relevant areas of the brain confirmed by computed tomography (CT) and/or magnetic resonance imaging (MRI). LAA stroke was diagnosed by experienced neurologists according to the TOAST classification [2]. Patients were enrolled if they: 1) aged 18 years or older; 2) were Southern Han Chinese; 3) had available blood samples. The following exclusion criteria were applied: the presence of other types of cerebrovascular disease (e.g. intracranial hemorrhage, subarachnoid hemorrhage, transient ischemic attack, cerebral aneurysm or cerebrovascular malformation), known embolic source (aortic arch, cardiac or carotid), severe systemic diseases such as neoplasms, autoimmune or inflammatory diseases, and serious chronic diseases (e.g. hepatic or renal failure). Three hundred symptom-free individuals were recruited as the control group. The inclusion criteria of control subjects were as follows: 1) aged 18 years or older; 2) were Southern Han Chinese; 3) had no history of cerebrovascular diseases; 4) had no history of atherosclerotic diseases; 5) had regular physical examinations. Exclusion criteria were the same as the criteria used for the patient group. The study was approved by the ethics committee of the First Affiliated Hospital of Guangxi Medical University and was carried out in accordance with the Declaration of Helsinki. All participants provided informed consent. All methods were performed in accordance with the relevant guidelines and regulations.

Genotyping
Genomic DNA was isolated from a 300 μL blood sample using the TIANamp Blood DNA Kit (Tiangen Biotect [Beijing] Co., China) according to the manufacturer's instructions. Extracted genomic DNA samples were stored at − 80°C until genotyping was performed. Two SNPs in GUCY1A3 gene, rs1842896 and rs7692387, were genotyped using the method of SNaPshot kit commercialized by Applied Biosystems, as described previously [12]. The sequences of primers used for polymerase chain reaction (PCR) and minisequencing extension were listed in Table 1.

Clinical characteristics of the study population
The clinical characteristics of LAA stroke patients and control subjects are summarized in Table 2 Table 2, the prevalence of conventional risk factors such as hypertension, diabetes mellitus, and cigarette smoking, was found to be higher in patients compared to the controls. Patients with LAA stroke had a higher prevalence of coronary artery disease history (8.05 vs. 3.67%) than controls. The level of HbA1c was significantly higher in LAA stroke patients when compared to controls.

Genotype distributions of the GUCY1A3 SNPs
The genotype distribution of the GUCY1A3 rs1842896 and rs7692387 in LAA stroke patients and controls are given in Table 3. The distribution of rs1842896 genotypes in LAA stroke patients significantly differed from the control group (P = 0.028), while the distribution of rs7692387 genotypes was similar between case and control individuals (P = 0.915). The TT, GT and GG genotype frequencies of rs1842896 were 54.36, 36.58 and 9.06% in patients, 44.67, 47.33, and 8.00% in controls, respectively. Obtained genotype frequencies for each SNP did not significantly deviate from Hardy-Weinberg equilibrium expectations (P > 0.05).

Associations between the GUCY1A3 SNPs and LAA stroke risk
We assessed the associations of the GUCY1A3 rs1842896 and rs7692387 polymorphisms with LAA stroke in different genetic models, including dominant, recessive, and additive models. Genotypic association between rs1842896 and LAA stroke was significant in the recessive model (recessive model: OR = 1.48, 95% CI: 1.07-2.04, P = 0.018, Table 4), there was no statistical significance in the other models (Table 4). After adjusted for age, gender, cigarette smoking, total cholesterol, low-density lipoprotein cholesterol,   Table 4). However, the rs7692387 polymorphism failed to show significant association in all three genetic models.

Discussion
We designed this study to investigate the associations between the GUCY1A3 polymorphisms and LAA stroke in Chinese Han population. Our results showed that, under the recessive model, the GUCY1A3 rs1842896 TT genotype was significantly correlated with LAA stroke. After adjustment for its effects on age, gender, cigarette smoking, total cholesterol, low-density lipoprotein cholesterol, HbA1c, hypertension, diabetes mellitus, and CAD, the rs1842896 TT genotype retained association with increased susceptibility to LAA stroke (recessive model: adjusted OR = 1.96, 95%CI: 1.22-3.17, P = 0.006), indicating the SNP might be associated with LAA stroke independently of its effects on hypertension, diabetes mellitus and CAD. This association suggested that the risk for LAA stroke in the rs1842896 TT genotype carriers were increased 1.96fold when compared with GG/GT genotype carriers. However, association between the rs7692387 polymorphism with LAA stroke was not observed. The rs1842896 is located 76.4 kb upstream of the GUCY1A3 locus. The GUCY1A3 gene encodes the α1 subunit of sGC, the major cellular receptor for NO that is implicated in pathogenesis of atherosclerosis. sGC acts as a heterodimer composed of an α subunit and a β subunit. Although two isoforms of each subunit (α1, α2, β1 and β2) exist, only the α1β1 heterodimer and the α2β1 heterodimer are functional enzymes [13]. The α1β1 heterodimer is the most abundant isoform distributed ubiquitously and displays higher activity than α2β1, so it is regarded as canonical sGC [14]. As a key enzyme of the NO signaling pathway, sGC can change the conversion of GTP into cGMP, leading to increased production of cGMP. Increasing intracellular levels of cGMP and the subsequent intracellular processes lead to relaxation of VSMCs and inhibition of platelet aggregation [14,15], preventing the formation of atherosclerosis. Previous studies have explored the therapeutic potential of sGC stimulators [16].
The impact of genetic variation in the GUCY1A3 gene on human atherosclerosis has been highlighted by previous studies. In 2012, a meta-analysis of two GWAS in Chinese Han population with 1515 CAD cases and 5019 controls identified rs1842896 at 4q32.1 near GUCY1A3 showed significant association with CAD [6]. Later, the CARDIoGRAMplusC4D Consortium [11] also detected a strong association between a variant at the GUCY1A3 locus and CAD. Recently, an extended meta-analysis [7] which considerably expanded the coverage of human genetic variation especially for low-frequency variants to determine whether these variants mediate risk for IS found that GUCY1A3 showed suggestive association with LAA stroke. Besides, it has been shown that rare coding variants in the GUCY1A3 gene were linked in families to myocardial infarction [17] and stroke [18] risk.
The risk variants in the GUCY1A3 gene leading to reduced sGC availability and activity may be one reason of increased risk in atherosclerotic events. Kessler et al. found GUCY1A3 gene variant was associated with both reduced platelet α1 sGC protein levels and increased atherosclerotic disease burden [19]. It has also been shown that absence or reduction of α1 subunit protein was detectable in variants in the GUCY1A3 gene identified in sporadic young myocardial infarction cases [20]. There are several limitations of our study that have to be taken into account. First, our study population was limited to southern Chinese individuals. As genetic influence is strongly relevant to ethnic background, our findings need to be validated in other ethnic populations. Second, our study sample size was relatively small. Third, biological functions were not conducted which were deserved further research. Fourth, it has been demonstrated that nutraceuticals and functional food ingredients can reduce the IS risk [21,22]. However, we were not able to collect the important risk factor for subjects who participated in the study. Further studies solving these limitations are needed.

Conclusion
To our knowledge, this is the first study to demonstrate an association between the GUCY1A3 rs1842896 and LAA stroke. We found that the GUCY1A3 rs1842896 TT genotype was associated with an increased susceptibility to LAA stroke in a Chinese Han population. This finding indicates a potential link between GUCY1A3 and risk of LAA stroke. Further research will be required to explore the role of GUCY1A3 in the pathogenesis of LAA stroke.