Rice bran oil could favorably ameliorate atherogenicity and insulin resistance indices among men with coronary artery disease: post hoc analysis of a randomized controlled trial

Background Despite recent advances in recognizing more reliable indicators to estimate the coronary artery disease (CAD) patients’ response to treatment and prognosis, less attention has been paid to evaluating them in clinical trials. Hence, the present research was conducted to study the impact of rice bran oil (RBO) versus sunflower oil (SFO) on various atherogenicity and insulin resistance markers. Methods In the present 8-week randomized controlled trial, 40 CAD men with an average age of 56 years were allocated randomly into the intervention or control group to use RBO or SFO (30 g/day) plus a standardized dietary plan. As a further analysis, eight atherosclerosis-related indices were calculated before and after the study. Results Analysis of covariance test in which potential confounders and baseline levels were considered, indicated that using RBO compared to SFO reduced Castelli’s risk index I and II (adjusted means:3.29, 1.52 vs. 4.61, 2.20, respectively), atherogenic coefficient (2.29 vs. 3.61), lipoprotein combine index (6.54 vs. 17.53), and cholesterol index (0.46 vs. 1.20) after the trial (P-value ≤ 0.002). Also, the RBO group yielded significantly lower triglyceride glucose index (8.73 vs. 9.13) (P-value = 0.010). Further, marginally significant amelioration in triglyceride/HDL ratio and atherogenic index of plasma (1.48 and 0.13 vs. 1.86 and 0.24 respectively) were noted (P-value = 0.07). Spearman correlation analysis detected significant positive correlations between alterations in TNF-α serum levels (ng/L) and the majority of evaluated indices (P-value < 0.05). Conclusion Taken together, incorporating 30 g of RBO into the patient’s usual diet appeared effective in ameliorating atherogenicity and insulin resistance indicators among men with CAD, probably in relation to its anti-inflammatory properties. Trial registration The protocol of the current trial was retrospectively recorded in the Iranian clinical trial registration system (IRCT) with the registration number of IRCT20190313043045N1 (URL: https://en.irct.ir/trial/38346; Registration date: 2019-04-27).


Introduction
As a chronic inflammatory condition of vessels, atherosclerosis is known to occur through the growth of lipidrich lesions, monocytes and macrophages accumulation over the vessels walls inner layer and releasing proinflammatory mediators which result in subsequent atheroma formation [1,2]. It is worth mentioning that males tended to have an augmented burden of severe atherosclerosis events by more than two times as much as females [3]. Conventionally, individualized lipid parameters in the serum, such as triglyceride, high-density lipoproteins-cholesterol (HDL-C), low-density lipoproteins-cholesterol (LDL-C), and total cholesterol, have been well-established as indicators of coronary artery disease (CAD) risk [4][5][6][7]. However, they yield limited information and would not be able to accurately reflect the severity of atherosclerotic lesions progression, arterial stiffness, and poor prognosis in CAD patients [5,[7][8][9][10][11][12][13][14]. Given that prompt and proper diagnosis of cardiovascular diseases (CVDs) modifiable risk factors, most importantly dyslipidemia, at early stages could prevent subsequent metabolic disorders, especially CAD, focusing solely on traditional lipid components, including LDL-C as a treatment target, has become a matter of debate [4-7, 10-12, 15]. Accordingly, during recent years, emerging pieces of evidence have proposed novel biomarkers such as lipoprotein(a) and atherosclerosisrelated indices [4-8, 11, 16, 17]. These indices which are mainly based on lipoprotein ratios would be able to better predict the risk of CAD, particularly among those having other risk factors. They seem to reflect small dense low-density lipoprotein (sdLDL) or LDL-C particles, and show more robust effects on atherosclerosis development and carotid artery stenoses [4][5][6][7][8]11]. Although the 2002 National Cholesterol Education Program guideline has mentioned sdLDL measurement could be considered as a complementary treatment target for patients with CAD and chronic metabolic disorders [18]; it, however, cannot be routinely assessed owing to being expensive, time-consuming, and difficult to measure [4][5][6][7][8]11]. Thus, several attempts have been made to identify surrogate markers of sdLDL and comprehensive atherosclerotic-related indices, including Castelli's risk indices I and II (CRI-I and CRI-II), lipoprotein combine index (LCI), atherogenic coefficient (AC), triglyceride to HDL-C ratio, triglyceride glucose (TyG) index, atherogenic index of plasma (AIP), and cholesterol index (CHOLINDEX) [19,20].
On the other hand, considering the renewed interest in incorporating more healthy functional food items into the patients' usual diet, much concern has been given to the rice bran oil (RBO) health advantages particularly in the past two decades. RBO contains a relatively high load of unsaturated fatty acids (43 and 22% poly-and mono-unsaturated fatty acids (PUFA and MUFA) [21][22][23]. Besides, a variety of further health advantages of RBO are ascribed to its nutraceutical elements [21,22,24,25]. Nevertheless, limited controlled trials are available concerning RBO impacts on various metabolic disorders. In the light of these pieces of evidence, recently, the current research team has conducted a randomized controlled trial (RCT) aiming to explore the metabolic capabilities of RBO compared to the conventional oil, sunflower oil (SFO), plus a standard diet, among the men suffering from CAD. It was delineated that RBO consumption resulted in improving left ventricular dysfunction and serum levels of metabolic and inflammatory markers and single lipid parameters, except for HDL-C [26]. Considering that the atherogenicity and insulin resistance indices mentioned above could further the present knowledge regarding atherosclerosis progression and yield a deeper understanding of lipoproteins balance, insulin resistance, and response to treatment among CAD patients, in the present research, therefore, it is attempted to extend the previous analysis. Hence, the efficacy of RBO in comparison with SFO on CRI-I, CRI-II, triglyceride to HDL-C ratio, TyG index, AC, AIP, LCI, and CHOLINDEX as novel indicators of metabolic abnormalities in males with coronary disease is explored.

Participants and intervention
The research design and data gathering details have been described elsewhere [26]. Concisely, forty male patients with the diagnosis of severe CAD following percutaneous coronary angioplasty in Heshmat Cardiovascular hospital, Guilan University of Medical Sciences (GUMS), were included in the present RCT from 2019 (April) to 2020 (September). Eligibility criteria, as described in Table 1, required individuals to be male, between 30 and 70 years, diagnosed with CAD, and having BMI less than 35 kg/m2. Patients who had any history of serious cardiovascular events, i.e., surgery, thromboembolism, acute coronary syndrome, cardiogenic shock or coronary artery bypass graft during the 6 months preceding the research, and those who had any comorbidities (i.e., kidney disorders, type 1 diabetes, concurrent viral infections, malignant diseases, or immune system-related disorders) were excluded from the trial. Also, those who had specific dietary habits or consumed dietary supplements during the month before the beginning of the study were excluded. Other exclusion criteria constituted suffering from abuse of alcohol or drugs, any alterations in the therapeutic approaches or medications throughout the trial, and disinclination to participate or keep on the study owing to any reason.
The trial was carried out within 3 days of angioplasty. Of 130 CAD patients the study interventional cardiologists initially examined, 40 individuals who fulfilled the inclusion criteria were assigned to each study arm to take A (RBO) or B (SFO) oil cans in a one-to-one randomization ratio (in blocks of four) using random permuted blocks. The four blocks were generated based on the lists of randomized codes (using the website http://www.randomization.com). Patients in the intervention or control arm were asked to eat 30 g RBO daily or the same amount of SFO, respectively, plus a standardized dietary plan accompanied by routine therapeutic approaches. All enrolled subjects in both RBO or SFO receiving groups were prescribed the same drugs. The RBO used in the present study was a product of Giltaz Company located in Rasht, Guilan province in Iran. The details on the fatty acid composition of the applied oils have been illustrated earlier [26].
The current trial design was confirmed by the Cardiovascular Diseases Research Center, GUMS, with the research number of 97,111,302, and carried out under the Declaration of Helsinki guideline. Also, the ethics committee of GUMS ascertained the study procedures with the ethics number of IR.GUMS.REC.1397.485. The protocol of this trial was retrospectively recorded in the Iranian clinical trial registration system (IRCT) with the registration number of IRCT20190313043045N1 (URL: https://en.irct.ir/trial/38346; Registration date: 2019-04-27). Because of the ethical issues, the usual drug therapies were continued for all studied subjects. All included individuals were provided informed consent in written format.

Data collection
At the initiation, the data on patients' demographic, socioeconomic and previous medical history was gathered via an in-person interview. Anthropometric data (i.e., height and weight) were evaluated at the initial visit and after the research. After that, the calculation of body mass index (BMI) was performed by dividing the value of weight (in kg) by height (in square meters). Besides, calculation of energy and macronutrient needs and the prescription of a standard customized healthy diet in accordance with the "United States Department of Agriculture (USDA) food guidelines for Americans, 2010" for all patients were performed by a registered dietitian.
At the first visit and post-intervention, fasting venous blood samples were collected from all enrolled individuals to assess lipid profile, fasting blood sugar (FBS), and serum inflammatory mediators (tumor necrosis factoralpha (TNF-α) in addition to high-sensitive C-reactive protein (hs-CRP)). More details on laboratory analysis have been reported in the previous publication [26].

Definition of the atherosclerosis related indices
The following formulas were applied to estimate the atherogenicity and insulin resistance indices in the current post-hoc analysis of RCT: [10,19].

Statistical methods
As it was indicated in previous report [26], the sample size of 20 patients in each study arm was calculated on the basis of considering 80% statistical power (α was set out at 0.05, and β was set out at 0.20) aiming to detect at least 30 mg/dL decrease in total cholesterol concentration with a drop-out rate of 15% (d = 30 and S = 31.58 mg/dL) [28]. For quantitative and categorical variables, the descriptive statistics were provided as mean and standard deviation (SD) or frequencies (%), respectively. After testing for normality applying the Kolmogorov-Smirnov a test, an independent sample t-test or Mann-Whitney U test was done to analyze between-group comparisons of quantitative variables. For categorical data, a chi-square test was conducted. Within-group alterations throughout the trial were also compared applying paired sample t-test or Wilcoxon signed-rank test. For investigating RBO impacts in comparison with SFO on atherogenicity and insulin resistance markers among the studied patients, analysis of covariance (ANCOVA) was run in which age, baseline values, and BMI levels were also considered. Accordingly, means (adjusted) and 95% confidence intervals (95% CI) were provided. Correlation analysis between alterations in TNF-α serum concentration (ng/L) and various indices over the 8 weeks duration of the present trial was explored by applying the Spearman correlation test. Statistical Package for Social Sciences software (version24, SPSS Inc., Chicago, USA) was applied for running statistical analysis. For statistical significance of the performed tests, P-value = 0.05 was set out.

Results
The participants recruitment procedure and baseline characteristics The studied subjects' recruitment procedure has been described elsewhere [26]. Briefly, from the 40 enrolled patients, 37 individuals completed the study. In total, 3 CAD patients were excluded from the RCT; of whom, one patient in the SFO group discontinued the trial due to starting a new dietary plan and two patients in the RBO group failed to complete the study owning to poor adherence to the intervention (n = 1) and moving to another city (n = 1). Thus, 18 patients received RBO (mean  Table 2 summarizes the features of the studied patients at the study initiation. The characteristics of both studied arms were comparable in terms of BMI, and age. The means (SD) of BMI of RBO and SFO receiving patients were about 27.00 and 26.08 and kg/m 2 , with no significant differences between groups (Table 2). No significant differences were noted in terms of the distribution of having a history of chronic disorders between the two studied arms. About half of the patients in SFO group and 35% of those in RBO group reported a history of cardiovascular disorders. Besides, approximately half of the subjects in both groups had a history of hypertension. Also, 32 and 10% of the control subjects and 47 and 29% of intervention group showed past medical history of hyperlipidemia and diabetes, respectively. All studied subjects were prescribed routine post angioplasty medications, including aspirin, clopidogrel (Plavix), atorvastatin, losartan, and pantoprazole.

Within-group changes in atherogenicity and insulin resistance markers
Regarding within-group changes in comparison with baseline values, the patients who consumed RBO demonstrated statistically significant reductions in the atherogenicity indices, including CRI-I (mean change compared to baseline: Besides, marginally significant decreases were noted in the triglyceride/HDL ratio (mean change compared to baseline: − 0.44 (0.92)) and AIP (mean change compared to baseline: − 0.11 (0.23); P-value ≤ 0.058) among the patients in the intervention arm; however, no significant alterations were indicated among SFO receiving patients except for AIP which showed a small increase (mean change compared to baseline: + 0.09 (0.16); P-value ≤ 0.034).
When comparing the mean changes between the studied groups over the 8-week period, significantly greater declines in CRI-I, CRI-II, triglyceride/HDL ratio, TyG index, AC, AIP, LCI, and CHOLINDEX (P-value ≤ 0.035) were detected among the patients in the intervention group (Table 3).

Correlation analysis between TNF-α levels and atherogenicity and insulin resistance markers
As it was observed in the previous report of this trial [26], RBO consumption resulted in attenuating inflammatory status as marked by serum TNF-α diminished levels, while SFO use was accompanied by a slight increment in the levels of TNF-α (ng/L). Accordingly, in the current analysis, the relationship between alterations in TNF-α serum concentration (ng/L) and various atherogenicity and insulin resistance indices before and after consuming RBO or SFO in addition to a standard diet was examined ( Fig. 1 (a-h)

Discussion
The present findings underline that incorporating 30 g per day of RBO as the main dietary fat source within a standard diet in comparison with the conventionally  Fig. 1 The Correlation between alterations in tumor necrosis factor-α (TNF-α) serum concentration and atherogenic indices levels throughout 8-week trial consumed oil (SFO) after 8 weeks tended to significantly enhance various atherogenicity and insulin resistance indices. As far as it is known, no similar clinical trials have been conducted to date. However, the current results, in agreement with the previous findings within the same trial [26], partly corroborate those of prior trials that evaluated the effects of RBO on serum glucose and conventional single lipid parameters and indicated the hypolipidemic and hypoglycemic effects of this oil [28][29][30][31][32][33][34][35]. Notably, the preliminary results of the same trial demonstrated that consuming 30 g RBO per day for 2 months resulted in improving left ventricular dysfunction and serum levels of single lipid parameters (i.e., total cholesterol, triglyceride, and LDL-C) in addition to inflammatory markers, blood sugar, and uric acid [26]. Concordant with these results, a recently published meta-analysis on eight clinical trials with 14 effect sizes found that consuming RBO seems to be effective in alleviating dyslipidemia risk to some extent since it led to enhancing serum levels of lipid components including triglyceride, total cholesterol, and LDL-C. However, as it was previously shown in this trial [26], this oil could not significantly change serum HDL-C levels [36]. Subgroup analysis for BMI also revealed that overweight individuals and those aged > 50 years experienced greater total cholesterol reductions than norm-weight or younger subjects [36]. An earlier meta-analysis on 11 RCT conducted by Jolfaie et al. also revealed similar results [37].
Among various atherosclerosis-related markers, AIP, introduced by Dobiasova et al. [20], can be estimated through the ten logarithmic transformations of the triglycerides to HDL-C ratio. AIP appeared to be wellcorrelated with LDL-C particles diameters, sdLDL level, and Intima-Media Thickness (IMT). Thus, it could alternatively serve as a strong indicator that well describe the burden of sdLDL cardiovascular complications [6-8, 13, 20, 38-40]. It is noteworthy that sdLDL, as a subcomponent of LDL-C with lower affinity to LDL-C receptors and higher potential for oxidizing and forming foam cells, appeared to be preferred to conventional lipid marker, LDL-C in predicting atherosclerosis risk or prognosis [4][5][6][7][8]11]. In the clinical setting, the application of AIP as a feasible measure to evaluate the antidyslipidemic potential efficacy of medications for preventing or treating obesity has been gaining wide attention [11,12,41]. Moreover, other atherogenicity indicators (i.e., CRI-I and CRI-II, AC, LCI, and CHOLINDEX) that were also observed to be improved following RBO consumption after controlling for baseline levels and confounding factors within the current trial, mainly incorporate the balance between proatherogenic and anti-atherogenic lipid components. In addition, they appeared to be particularly of importance in research on developing novel agents for CVDs treatment [4,9,19,20,39].
RBO appeared to be an ideal sample of antiatherogenic functional food with lipid reducing, antiinflammatory, and anti-oxidant features, as it is not substantially changed during oxidative stress owning to its unsaponifiable fraction and relatively high MUFA content [21,22,24,25]. This heart-friendly oil has been shown to play a favorable role in controlling plasma lipid profile, particularly amongst individuals who have diabetes [21,42]. Oleic acid makes up 38% of the RBO fatty acid profile, and linoleic and linolenic acids form 34 and 2.2%, respectively [21][22][23]. Although the exact mechanisms through which RBO could exert antihyperlipidemic and anti-hyperglycemic effects are remained to be fully described, its nutraceutical composition can partly explain these beneficial effects. It has been evident that the plant sterols and γ-oryzanol content of RBO in addition to its vitamin E and tocotrienol constituent, which show anti-atherogenic and antioxidant activities, may predominantly contribute to ameliorating hyperlipidemia, hypercholesterolemia, hyperglycemia, hyperinsulinemia, inflammation and lipid peroxidation. Of note, γ-oryzanol constituent of RBO was also indicated to diminish the concentration of plasma non-HDL cholesterol levels, possibly via promoting the release of bile acids and cholesterol in the feces [33,35,37,[43][44][45][46][47][48][49][50][51][52][53]. The available evidence indicates that RBO might reduce triglyceride concentration by averting the formation of very low-density lipoprotein cholesterol (VLDL-C) and the lipoproteins that contain apolipoprotein-B100. In addition, RBO could modulate the lipoprotein lipase activity and trigger triglyceriderich lipoproteins breakdown. In particular, based on the experimental research on hyperlipidemic animals, RBO tocotrienol rich fraction was proved to notably restrain the activity of the main enzyme contributed to the cholesterol metabolism, 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-COA) reductase, and through this way, could attenuate hypercholesterolemia in a dosedependent manner [37,44,47,53]. It has also been mentioned that γ-oryzanol, in addition to tocotrienols, might promote cholesterol 7-alpha-hydroxylase (CYP7A1), the enzyme with rate restriction ability in the synthesis of cholesterol, which stabilizes serum cholesterol concentration by modulating cholesterol-bile acids conversion [33,37,53]. With respect to these mechanisms, RBO has also been shown to ameliorate insulin resistance and attenuate lipotoxicity and glucotoxicity [32, 35, 37, 43-46, 48, 54].
Moreover, the TyG index, another proposed indicator of atherosclerosis, is shown to be directly related to lipotoxicity, glucotoxicity, and an increased risk of metabolically unhealthy conditions, including CAD, diabetes, fatty liver arterial stiffness, hypertension, and obesity [7,27,55]. In particular, the TyG index that integrates serum fasting concentrations of triglycerides and glucose, has been broadly utilized as a substitute index for exploring resistance to insulin instead of Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) in the medical settings. Proposing a surrogate marker for insulin resistance would be helpful, given that HOMA-IR seems to be costly and time-consuming, and insulin serum concentrations measurement is required to be incorporated in [27,56,57]. Concerning this, in the current report, significant reductions in TyG index levels were detected after consuming 30 g of RBO for 8 weeks. Hence, it can be hypothesized that RBO intake could also result in attenuating insulin resistance. The antihyperglycemic and insulin resistance improving effects of RBO could also be attributed to its oleic acid content of this oil [32]. Also, the decrease in the liver triglyceride accumulation could play a role in alleviating insulin resistance following RBO, probably owing to its high MUFA content. However, no exact mechanism has yet been proposed for these anti-hyperinsulinemic effects of RBO [53]. From a more detailed perspective, the available experimental research revealed that adding RBO to the diet of rats as the only source of fat seems to decrease HOMA-IR and insulin level, and refine sensitivity to insulin certainly by stimulating the expression of involved genes, including glucose transporters-4 and 5 (GLUT-4 and GLUT-5) in addition to enhancing insulin receptor substrate-1, and insulin receptor activity [58].
Additionally, the positive correlations found between alterations in atherogenicity and insulin resistance markers and TNF-α concentration (ng/L) in the serum were among the interesting findings of the current analysis. These correlations drew our attention to the concept that anti-atherogenic and insulin resistance ameliorating effects of RBO might be partly due to its influences on suppressing inflammation and combating the oxidative stress. As reported in the available evidence, RBO and/or its main components administration seemed to decline the amounts of pro-inflammatory and pro-oxidative mediators (for example, CRP, interleukin (IL)-1b, TNF-α, IL-6, and malondialdehyde). On the flip side, it appeared to elevate the concentration of antiinflammatory mediators (for example IL-10, IL-4, superoxide dismutase, and catalase) [33,[48][49][50][51][52]. It is noteworthy that RBO and particularly γ-oryzanol and tocotrienol exert antioxidant and anti-inflammatory effects mainly through disturbing nuclear factor-kappa B (NF-κB) p65 pathway and suppressing the release of vascular endothelial growth factors. Besides, these components may play a role in increasing the expression of sterol regulatory peroxisome proliferator-activated receptor gamma (PPARγ) and element-binding protein (SREBP)-2 which could ultimately lead to alleviation of atherosclerosis progression [33,[48][49][50][51][52]59]. However, the link between atherosclerosis-related markers and inflammatory cytokines as well as the mechanisms by that RBO could favorably affect metabolic and inflammatory status remained to be elucidated more broadly.
Comparisons with other studies and what does the current work add to the existing knowledge During previous years, much more consideration has focused on the provision of surrogate markers of sdLDL as well as the suggestion of lipid ratios that comprehensively delineate the balance between lipoproteins with atherogenic and antiatherogenic potentials. These indicators seem to be more practical than traditional single lipid parameters. They could further the present knowledge regarding the early prediction of CAD alongside the effectiveness of the treatment strategies even when other lipid parameters remain within normal ranges [4,5,[8][9][10][11]38]. However, despite these advances, less attention has been paid to evaluating these indicators in clinical trials exploring the effects of pharmacological or dietary factors on CVDs progression. As far as it is known, there has been no similar RCT in which the changes in atherogenic indices following RBO consumption are investigated. This post hoc analysis of a previously performed RCT, therefore, seems to be the first study to consider these objectives. It was revealed that RBO as the main dietary fat source within a standard diet could significantly enhance atherogenicity and insulin resistance by decreased levels of CRI-I, CRI-II, AC, LCI, CHOLINDEX, and TyG index. RBO also leads to marginally significant enhancements in triglyceride to HDL-C ratio and AIP levels. Additionally, to determine whether RBO could improve the atherogenicity and insulin resistance status through attenuation in inflammation or not, the correlation of alterations in TNF-α serum concentration (ng/L) and various indices over the 8 weeks duration of the trial was examined. Significant correlations were detected between changes in the majority of atherogenicity and insulin resistance markers such as CRI-I, CRI-II, TyG index, AC, LCI, triglyceride to HDL-C ratio, and AIP and serum inflammatory status as measured by TNF-α (ng/L). Hence, it might be speculated that the RBO potential in suppressing inflammation seems to be the key mechanism that contributed to its anti-atherogenic influences. Therefore, encouraging CAD patients to consider this heart-friendly vegetable oil as the main source of daily fat would attenuate atherosclerosis progression and its related metabolic disorders. However, more experimental and clinical trials are required to confirm these results and explore the underlying mechanisms.

Study strength and limitations
To the best of our knowledge, it is the first time that a controlled trial has been conducted on men with CAD aiming to explore the effects of RBO on serum atherogenicity and insulin resistance indices instead of conventional individualized lipid components. These indicators appear to reflect the balance between lipoproteins, depict the patients' response to the treatment, and explore CAD prognosis better than the traditional single lipid parameters. Therefore, they would be important in the research on the efficacy of novel interventions for CAD treatment. On the other hand, applying the proposed atherosclerosis-associated indicators as treatment targets in the clinical setting seems to improve the clinicians' insight into patients' responses to treatment. Although the present findings seem promising, considering the fact that only male subjects with CAD were included in this RCT, caution must be applied when interpreting the results as the observed effects might not be simply generalized. Moreover, the small sample size and the posthoc nature of the study are among the other potential limitations of the current findings. Further, considering ethical issues, it was not possible to remove the routine drug therapy of included patients, thereby, the confounding effects of concurrently prescribed medications could not be excluded. However, to minimize the confounding effects of concurrently administered medications, they were prescribed in a similar manner for participants in both studied arms. Besides, regarding other confounders, it was tried to consider the effects of age and BMI as the confounders using ANCOVA though there might be additional confounding variables that can influence RBO effects on outcomes in CAD. Therefore, in the future trials the assessment and adjustment of these factors as well as including female participants should be taken into the account.

Conclusion
Taken together, incorporating 30 g of RBO to the patient's usual diet compared to the conventionally consumed oil, SFO, for 2 months appeared effective in improving atherogenicity and insulin resistance. These improvements were indicated by enhanced values of CRI-I and II, AC, LCI, CHOLINDEX, triglyceride to HDL-C ratio, AIP and TyG index following RBO consumption among men with CAD. The anti-atherogenic ameliorating effects of RBO might be partly related to its anti-inflammatory features considering the positive correlations between changes in the indices and serum TNF-a levels (ng/L). without any involvement in different stages of the research procedures, including data gathering or interpretation of the findings or publication. Besides, the authors did not have any affiliation with this company. We also would like to express our gratitude to the participants of the study and our colleagues, Dr. Zahra Nikpey, Ms. Motahare Haghighatkhah, Dr. Aboozar Fakhr mousavi, Dr. Mahboobeh Gholipour, and Dr. Amir Pourfarzad, for their kind cooperation in data collection.

Availability of data and materials
The analyzed datasets are available from the corresponding author upon rational request.

Declarations
Ethics approval and consent to participate The current trial design was confirmed by the Cardiovascular Diseases Research Center, GUMS, with the research number of 97111302, and carried out under the Declaration of Helsinki guideline. Also, the ethics committee of GUMS ascertained the study procedures with the ethic number of IR.GUMS.REC.1397.485. Because of the ethical issues, the usual drug therapies were continued for all studied subjects. All included individuals were provided informed consent in written format.

Consent for publication
Not applicable.