Figure 6
Effects of dietary supplementation with various compounds on the secretion of TNF-α by thioglycolate-elicited peritoneal macrophages stimulated with LPS. 4-week-old and 42-week-old C57BL/6 male mice were fed control diet or control diet supplemented with quercetin, δ-tocotrienol or dexamethasone for 4 week. After this, thioglycolate-elicited peritoneal macrophages were collected, adhered to the bottom of 12 well plates (1×107 cells/well in 1.0 mL medium) for 4 h, then washed with medium three times. Then cells were treated with LPS (100 ng/well), and incubated at 37°C in 5% CO2 for 4 h [9]. Supernatants were used to carry out TNF-α assays by ELISA. Data are means ± SD; n = 3 mice per group, with triplicate analysis of each sample. Values in columns with different symbols differ at P< 0.05. The treatments 1 - 8 correspond to: 1 - 4 = 8-week-old mice or 5 - 8 = 46-week-old mice treated with 100 ng LPS. CD = control diet; Quer = quercetin; Trie = δ-tocotrienol; Dexa = dexamethasone.