Inhibition of nitric oxide in LPS-stimulated macrophages of young and senescent mice by δ-tocotrienol and quercetin

Table 7 Effects of LPS, or LPS + with and without quercetin (Quer), δ-tocotrienol (δ-Trie) or dexamethasone (Dexa) on canonicall pathways in liver samples of C57BL/6 male mice.

#	Functions	LPS	LPS + Quer	Quer	LPS + δ-Trie	δ-Trie	LPS + Dexa	Dexa
1	C21-Steroid Hormone Metabolism	11			9	8
2	Notch Signaling	25		19	23	20	22
3	Serotonin Receptor Signaling	14		13	13	10	11	11
4	Tyrosine Metabolism	21	20		23	16
5	Taurine and Hypotaurine Metabolism	7	6	6	7	6	6
6	Retinol Metabolism	5			5	5	4
7	Toll-like Receptor Signaling	31	24		31	30
8	Bile Acid Synthesis	20
9	Histidine Metabolism	16
10	Nitric Oxide Signaling in Cardiovascular					27
11	FGF Signaling			32
12	GABA Receptor Signaling				24	21		22
13	Dopamine Receptor Signaling			20		19
14	Sulfur Metabolism		5	4			5	4
15	Valine, Leucine, Isoleucine Degradation				17		6
16	Alanine and Aspartate Metabolism		16
17	Tryptophan Metabolism			35	11	43	39
18	Phenylalanine, Tyrosine Biosynthesis				15
19	Cysteine Metabolism			7	7	7
20	Pantothenate & CoA Biosynthesis				34	30
21	Nicotinate & Nicotinamide Metabolism		16
22	Prostaglandin & Leukotriene Metabolism		30	26
23	Fatty Acid Biosynthesis				5	4		4

¹The data in this Table represents the expression of a number of genes modulated by various treatments. Macrophages were treated with LPS (10 ng/well), quercetin (40 μM), δ-tocotrienol (20 μM), or dexamethasone (10 μM) with and without LPS (10 ng/well) as described in the experimental section. Total RNA was extracted from the various treated cells and their gene expression profiles compared with the control (LPS), and untreated cells. The values here have been corrected for differences in the arrays. The gene expression values are reported as average normalization log ratios. A data set containing gene identifiers and their corresponding expression values were uploaded as an Excel spreadsheet using the template provided in the Ingenuity Pathway Analysis programm Various functions, their location, and gene expression values in this table represent for inhibitors only. The details of comparison for specific gene expression for each inhibitor were reported in Tables 8,9,10.

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