Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue

Table 2 Expression in subcutaneous adipose tissue of ceramide-metabolising enzymes in relation to adipose tissue inflammation

Gene	ABI assay ID	Ct value	Less inflamed adipose tissue, normal liver fat	More inflamed adipose tissue, high liver fat	P
			n = 10	n = 10
SPTLC1	Hs00272311_m1	27.2	0.99 ± 0.04	1.05 ± 0.06	0.20
SPTLC2	Hs00191585_m1	26.8	0.97 ± 0.07	1.00 ± 0.05	0.39
DEGS1	Hs00186447_m1	25.9	0.99 ± 0.05	1.07 ± 0.05	0.11
LASS1	Hs00242151_m1	33.2	9.07 ± 3.12	9.22 ± 1.63	0.48
LASS4	Hs00226114_m1	27.1	1.08 ± 0.10	1.07 ± 0.16	0.49
LASS6	Hs00826756_m1	28.8	0.87 ± 0.07	0.89 ± 0.07	0.42
ASAH1	Hs00602774_m1	24.3	1.16 ± 0.10	1.47 ± 0.13	0.03
UGCG	Hs00234293_m1	28.2	0.91 ± 0.08	0.96 ± 0.06	0.34
SGMS1	Hs00380453_m1	28.0	1.01 ± 0.05	1.10 ± 0.06	0.12
SGMS2	Hs00398067_m1	30.6	2.09 ± 0.27	2.57 ± 0.32	0.13
SMPD1	Hs00609415_m1	26.6	1.51 ± 0.10	1.85 ± 0.10	0.01 ^a
SMPD2	Hs00162006_m1	29.8	1.13 ± 0.06	1.26 ± 0.06	0.08 ^a
SMPD3	Hs00218713_m1	33.8	1.33 ± 0.13	1.76 ± 0.18	0.05 ^a
SMPD4	Hs00215775_m1	29.1	1.57 ± 0.09	1.52 ± 0.09	0.35
CERK	Hs00368483_m1	29.3	1.57 ± 0.15	1.87 ± 0.10	0.06
SPHK1	Hs00184211_m1	28.9	12.18 ± 1.49	17.64 ± 1.65	0.01
CGT	Hs00409961_m1	35.5	4.45 ± 1.29	3.45 ± 0.52	0.24
CDH5	Hs00174344_m1	28.3	1.16 ± 0.14	1.43 ± 0.13	0.09
SELE	Hs00950401_m1	33.2	0.72 ± 0.15	2.45 ± 0.94	0.04
VEGF	Hs0090054_m1	26.4	4.13 ± 0.37	3.71 ± 0.52	0.25
HIF-1α	Hs00153153_m1	27.6	1.39 ± 0.12	1.65 ± 0.08	0.02

Relative gene expression levels of genes involved in ceramide and sphingomyelin metabolism in subcutaneous adipose tissue of obese women with different degrees of adipose tissue inflammation are given. SPTLC: serine palmitoyl transferase long-chain; DEGS: dihydroceramide desaturase; LASS: LAG1 homolog (ceramide synthase); ASAH: N-acylsphingosine amidohydrolase (ceramidase); UGCG: UDP-glucose ceramide glucosyltransferase; SGMS: sphingomyelin synthase; SMPD: sphingomyelin phosphodiesterase (sphingomyelinase); CERK: ceramide kinase; SPHK: sphingosine kinase; CGT: ceramide glycosyl transferase; CDH5: cadherin 5 (vascular endothelium); SELE: selectin E; VEGF: vascular endothelial growth factor; HIF-1α: hypoxia-inducible factor 1α. Gene expression was normalized to housekeeping genes RPLP0 and TBP, which did not differ between the groups (for RPLP0: 1.94 ± 0.26 vs 2.11 ± 0.17, NS; for TBP: 3.96 ± 0.48 vs 3.97 ± 0.44, NS). Values are expressed as mean ± SEM. ^a Reported previously [3].

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