Figure 4

The effect of oligomycin (10 μM; 3 h) on A) fold-change of genes involved in cholesterol metabolism and lipoprotein signalling in RAW 264.7 macrophages, compared with control cells, as judged by Q-PCR analysis of genes implicated in the cholesterol efflux, biosynthesis and esterification pathways; B) incorporation of [³H]oleate (10 μM) into the cholesteryl ester pool, measured in the presence of absence of acetylated LDL (50 μg ml^-1; 3 h); C) incorporation of [¹⁴C]acetate into free cholesterol, cholesteryl ester, fatty acids, phospholipids and triacylglycerol (3 h) and D) induction of apoptosis in RAW 264.7 murine macrophages. Values are the mean±SEM of between three to six independent experiments. *p<0.05, **p<0.01 and ***p<0.001 compared with control macrophages.