Table 2 The primer Sequences Used for PCR Amplification
From: Effect of H2S on the circadian rhythm of mouse hepatocytes
Gene | Genebank Accession No. | Annealing temperature | Primer sequence 5' to 3' |
|---|---|---|---|
Clock | NM_007715 | 58°C | Forward:CTTCCTGGTAACGCGAGAAAG Reverse:TCGAATCTCACTAGCATCTGACT |
Bmal1 | NM_007489 | 60°C | Forward:CACTGACTACCAAGAAAGTATG Reverse:ATCCATCTGCTGCCCTGAGA |
Per2 | NM_011066 | 58°C | Forward:CAGACTCATGATGACAGAGG Reverse:GAGATGTACAGGATCTTCCC |
Rev-erbα | NM_145434 | 60°C | Forward:TACATTGGCTCTAGTGGCTCC Reverse:CAGTAGGTGATGGTGGGAAGTA |
Sirt1 | NM_019812 | 58°C | Forward:GAACAGGTTGCGGGAATC Reverse:AACATGAAGAGGTGTGGGTG |
GAPDH | BC_083149 | 60°C | Forward:ACAGCCGCATCTTCTTGTGCAGTA Reverse:GGCCTTGACTGTGCCGTGAATTT |