Figure 3

A. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were treated with 1mM Aspirin. At 24 hours post Aspirin treatment, supernatants and cellular extracts were collected; total incubation time was 48 hours. Cellular extracts were assayed for total Glutathione content. Values were normalized to the untreated. Untrtd = Untreated; Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). * = p< 0.05; ** = p<0.005, #### = p<0.00005. 3B. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were treated with Poly I:C, 1mM Aspirin, or a combination of Poly I:C and Aspirin. At 24 hours post Poly I:C and/or Aspirin treatment, supernatants and cellular extracts were collected; total incubation time was 48 hours. Cellular extracts were assayed for total Glutathione content. Values were normalized to the untreated. Untrtd = Untreated; PIC = Poly I:C (1μg/mL); Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). * = p<0.05; **,##,§§ = p<0.005. 3C. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were treated with Imiquimod, 1mM Aspirin, or a combination of Imiquimod and Aspirin. At 24 hours post Imiquimod and/or Aspirin treatment, supernatants and cellular extracts were collected; total incubation time was 48 hours. Cellular extracts were assayed for total Glutathione content. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); Asp = Aspirin (1mM); DHA = Docosahexaenoic Acid (50μM). *,#,§ = p<0.05; ** = p<0.005.