Figure 4

A. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with TLR agonists. At 24 hours post TLR stimulation, supernatants were collected; total incubation time was 48 hours. IL-6 secretion was determined by assaying the supernatants with an IL-6 ELISA. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); PIC = Poly I:C (1μg/mL); DHA = Docosahexaenoic Acid (50μM). *,# = p< 0.05. 4B. 50,000 EOC20 Microglia were plated in a 96 well plate containing Complete Medium plus 20% LADMAC Conditioned Medium. Cells were stimulated with 50μM DHA for 24 hours. After 24 hours, cells were stimulated with TLR agonists. At 24 hours post TLR stimulation, supernatants were collected; total incubation time was 48 hours. TNF- α secretion was determined by assaying the supernatants with a TNF- α ELISA. Values were normalized to the untreated. Untrtd = Untreated; Imi = Imiquimod (10μg/mL); PIC = Poly I:C (1μg/mL); DHA = Docosahexaenoic Acid (50μM). *,# = p< 0.05.