Table 1 qPCR oligonucleotides and RT-qPCR efficiencies for Caco-2 cells
From: PPARγ as a sensor of lipase activity and a target for the lipase inhibitor orlistat
Gene name /(genebank accession no.) | Primer and probe sequences |
|---|---|
GAPDH: Glyceraldehyde-3-phosphate dehydrogenase (NM_002046.3) | F: AGCCACATCGCTCAGACAC |
R: GCCCAATACGACCAAATCC | |
ACTB, β-actin (NM_005345.4) | F: GGAGTCCTACGCCTTCAACA |
R: CCAGCACCTTCTTCTTGTCG | |
hBD1, Human β defensin 1 (X92744.1) | F: TGTCTGAGATGGCCTCAG GT |
R: GGGCAGGCAGAATAGAGACA | |
ADRP, Adipose Differentiation Related Protein (NM_001122.2) | F: TCAGCTCCATTCTACTGTTCACC |
R: CCTGAATTTTCTGATTGGCACT | |
PPARγ variant 1 (NM_138712.3) | F: GACAGGAAAGACAACAGACAAATC |
R: GGGGTGATGTGTTTGAACTTG |