Figure 1

Insulin stimulated glucose uptake in isolated soleus muscle. Data are presented as mean plus standard error. Dashed line represents average basal (unstimulated) glucose uptake in all groups. *significantly different from basal (unstimulated) glucose uptake (p < 0.05). SOL strips were equilibrated for 30 minutes in gassed KHB (0.1% fatty acid free BSA) at 30C containing 8 mM glucose and 32 mM mannitol, in the absence or presence of insulin (10 mU/mL). Strips were washed twice (10 min each) with glucose-free KHB (4 mM pyruvate, 36 mM mannitol). After washing, SOL strips were incubated for 20 min (insulin-stimulated, 10 mU/mL) or 40 min (basal) in KHB (4 mM pyruvate, 8 mM 3-O-[³H] methyl-D-glucose (800 μCi/mmol), 28 mM [¹⁴C]mannitol (60 μCi/mmol)].