Table 6 Primers for analysis of transcription level of gene expression
Gene | Accession no. | Primer | Tm, °C | Amplicon size,bp |
|---|---|---|---|---|
PPAR-α | NM_013196.1 | F 5'-GGTCATACTCGCAGGAAAG-3' | 53 | 151 |
R 5'- GCAGCAGTGGAAGAATCG-3' | ||||
CPT1A | NM_031559.2 | F 5'-GCCAGACGAAGAACATTG-3' | 53 | 171 |
R 5'-CCTTGACCATAGCCATCC-3' | ||||
ACC | NM_022193 | F 5'-TTGGTGCTTATATTGTGGATGG-3' | 50 | 127 |
R 5'-ATGTGCCGAGGATTGATGG-3' | ||||
FASN | NM_017332.1 | F 5'-GGTAGGCTTGGTGAACTGTCTC-3' | 55 | 201 |
R 5'-TCTAACTGGAAGTGACGGAAGG-3' | ||||
SREBP-1 | XM_213329 | F 5'-CATCAACAACCAAGACAGTG-3' | 50 | 126 |
R 5'-GAAGCAGGAGAAGAGAAGC-3' | ||||
ACTIN | NM_031144.2 | F 5'–CCC ATC TAT GAG GGT TAC GC-3' | 50 | 150 |
R 5'-TTT AAT GTC ACG CAC GAT TTC-3' |