Figure 1From: LDL-cholesterol signaling induces breast cancer proliferation and invasionProliferation, migration and loss of adhesion induced by LDL in in breast cancer cell lines. A, Number of cells (MDA MB 231, HTB 126, HTB 20) after 24 h and 48 h of LDL (100 μg/mg) exposure is consistently higher than control (non-treated) condition. B and C, Cells (MDA MB 231) were wounded and then cultured for 24 h, with LDL (100 μg/mL), HDL (100 μg/mL) or control conditions. Cell migration into the wound was examined by phase-contrast microscopy and migration distance is indicated as the percentage of the wound closure at 24 h. Representative photos are shown (original magnification 100x C). D and E, Number of cells (MDA MB 231) adherent and no adherent cells, at 4 h after being removed and reseeded on its primary conditions (control and LDL100 μg/mL) shows that LDL treated cells lose matrix adhesion compared to control. Representative photos are shown (original magnification 200x E). P value and the number of the experiments are represented in the figure. Columns mean; bars ± SEM.Back to article page