Figure 1

Dose- and time-related changes of IL8 mRNA expression following free fatty acid treatment. 1a. Expression of IL8 following 500 μM palmitate treatment, showing the highest expression with 9 hours of treatment (92.1 fold greater than the control, Huh7 cells incubated with bovine serum albumin; n = 4, *p < 0.0001). b. Expression of IL8 after 9 hours of treatment with palmitate, showing dose-related changes with the highest expression of IL8 following treatment with 500 μM palmitate (104.9 fold greater than the control, Huh7 cells incubated with bovine serum albumin; n = 4, *p < 0.01). c. Expression of IL8 after 9 hours of treatment with linoleate, showing dose-related changes. Expression of IL8 was 5.2 to 11.7 fold increase from 100 to 800 μM, however, it increased markedly at 900 μM with a 53.8 fold increase and 1000 μM with a 41.9 fold increase (n = 4, *p < 0.01).