N-3 fatty acid intake altered fat content and fatty acid distribution in chicken breast muscle, but did not influence mRNA expression of lipid-related enzymes

Table 2 Gene expression analyses

Gene	Accession no.	Forward (5′-3′)	Reverse (5′-3′)	Amplicon (bp)
fads1	XM_420557	GAGCCATCGGTGAGGGTTTC	CTCCAGTCCTTTCCTTGCGT	186
fads2	NM_001160428	ACTGGTGGAACCATCGTCAC	GCAGAGGTGGGAAGATGAGG	189
fads9	NM_204890	GGAGCCCTAGGAGAAGGTTTC	AAATTGAAGCGCCAGCCAAA	86
hmgcr	NM_204485	TGTTGTAAGGCTGCCCTCTG	TAGGCGGGCAAACCTACTTG	118
cpt1	DQ314726	TGACGTCGATTTCTGCTGCT	GCAGCGCGATCTGAATGAAG	98
acox1	NM_001006205	CCAGTCAGCTTGGTAGAGGC	AGTGACAGTGTGCCTCAGATG	160
actB	NM_205518	AAATCAAGATCATTGCCCCACCT	AGGGGTGTGGGTGTTGGTAA	181
gapdh	NM_204305	GCTAAGGCTGTGGGGAAAGT	TCAGCAGCAGCCTTCACTAC	161
rpl4	NM_001007479	TGTTTGCCCCAACCAAGACT	TCCTCAATGCGGTGACCTTT	136
ywhaz	NM_001031343	AGGAGCCCGTAGGTCATCTT	TCCAACAGAGACAGCACATCA	150
gpx4	AF498316	CAGTACAGGGGCTTCGTCTG	CAGCCCCTTCTCAGCGTATC	114
sodmt	NM_204211	CGCTGGCAAAAGGTGATGTT	CTCCTTTAGGCTCCCCTCCT	132

ISSN: 1476-511X