Figure 6From: Syndecan-1 mediates internalization of apoE-VLDL through a low density lipoprotein receptor-related protein (LRP)-independent, non-clathrin-mediated pathwayHeparin, but not RAP-GST, competes for DiI-apoE-VLDL uptake by GM00701/Syn-1-HA cells. GM00701/Syn-1-HA cells were cultured on glass coverslips and incubated with DiI-labeled apoE-VLDL (4 μg/ml) in the absence (panel A) or presence of recombinant human RAP-GST (50 μg/ml, panel B) or heparin (200 μg/ml, panel C) at 37°C for 3 h. Cells were fixed and processed for fluorescence microscopy. Magnification, 630X.Back to article page