Uptake of the glycosphingolipid sulfatide in the gastrointestinal tract and pancreas in vivo and in isolated islets of Langerhans

Table 2 Sulfatide in the intestine, stomach and liver after oral administration of ³H-sulfatide

Hours after sulfatide load	Stomach nmol/mg protein	Duodenum/Jejunum nmol/mg protein	Ileum nmol/mg protein	Terminal ileum nmol/mg protein	Liver nmol/mg protein
1–2	0,58 ± 0,14 (8)	0,09 ± 0,01 (8)	0,24 ± 0,02 (8)	0,61 ± 0,26 (3)	0,004 ± 0,001 (5)
5–6	0,18 ± 0,03 (7)*	0,04 ± 0,01 (7)*	0,08 ± 0,02 (7)**	0,08 ± 0,03 (7) (n.s.)	0,004 ± 0,001 (7) (n.s)
24	0,26 ± 0,04 (3)*	0,03 ± 0,01 (7)*	0,05 ± 0,01 (7)**	0,05 ± 0,01 (7) (n.s.)	0,006 ± 0,003 (3) (n.s.)

BALB/c mice received sulfatide (250 nmol-1 μmol/mL, 1.7 × 10⁶ dpm/mouse of ³H-sulfatide) orally, whereafter the animals were sacrificed after 1–2 hours (n = 8), 5–6 hours (n = 7) or 24 hours (n = 7). Values are mean ± SEM (n). Sulfatide was quantified using TLC-ELISA.
* p < 0.05; ** p < 0.01, with respect to the sulfatide concentrations 1–2 hours after sulfatide administration. n.s. = not significant.

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