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Figure 3 | Lipids in Health and Disease

Figure 3

From: Synthesis of the oxysterol, 24(S), 25-epoxycholesterol, parallels cholesterol production and may protect against cellular accumulation of newly-synthesized cholesterol

Figure 3

Endogenously-produced 24,25EC correlates with cholesterol synthesis when cholesterol synthesis is stimulated by statin-pre-treatment. (A) CHO-7 cells were incubated with the indicated concentrations of compactin in the presence of [1-14C]-acetate for 24 h. Neutral lipid extracts were separated by thin-layer chromatography and bands corresponding to authentic cholesterol and 24,25EC were visualized by phosphorimager and quantified by densitometry. Values are means+SEM from 3 separate experiments. (B, C) CHO-7 cells were pre-incubated with the indicated concentrations of compactin for 24 h. (B) mRNA levels for HMG-CoA reductase was measured using QRT-PCR. Data are presented relative to vehicle-treated controls and are means+SEM (3 replicate cultures representative of 2 separate experiments). (C) Cells were then incubated in the presence of [1-14C]-acetate for 2 h. Neutral lipid extracts were separated by thin-layer chromatography and bands corresponding to authentic cholesterol and 24,25EC were visualized by phosphorimager and quantified by densitometry. Equations for the lines of best fit (D) y = 1.47x - 0.42; R2 = 0.99; P < 0.001. Values are means from 2 separate experiments set relative to the vehicle-treated control condition which was assigned an arbitrary value of 1.

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Lipids in Health and Disease

ISSN: 1476-511X