Genetic and diet effects on Ppar-α and Ppar-γ signaling pathways in the Berlin Fat Mouse Inbred line with genetic predisposition for obesity

Wagener, Asja; Goessling, Helge F; Schmitt, Armin O; Mauel, Susanne; Gruber, Achim D; Reinhardt, Richard; Brockmann, Gudrun A

doi:10.1186/1476-511X-9-99

Lipids in Health and Disease

Table 2 Changes in expression of genes involved in the PPAR pathway.

From: Genetic and diet effects on Ppar-α and Ppar-γ signaling pathways in the Berlin Fat Mouse Inbred line with genetic predisposition for obesity

	Gene symbol	BFMI860/B6 (SMD)				HFD/SMD (BFMI860)
Entrez Gene-IDs		Fat		liver		fat		liver
		Fold ch.	p-value	Fold ch.	p-value	Fold ch.	p-value	Fold ch.	p-value
PPAR isoforms and receptors
19013	Ppara	0.87	< 0.01	2.67	< 0.01	0.95	ns	0.48	< 0.01
19015	Ppard	1.07	ns	0.98	ns	0.73	ns	0.88	ns
19016	Pparg	0.66	< 0.01	0.83	< 0.05	0.70	< 0.01	0.96	ns
20181	Rxra	0.69	< 0.01	1.09	< 0.05	0.95	ns	0.89	< 0.05
20182	Rxrb	0.98	ns	1.06	ns	1.00	ns	1.00	ns
20183	Rxrg	0.84	< 0.01	1.29	< 0.01	0.81	< 0.01	1.02	ns
Lipid transport
11806	Apoa1	0.97	ns	0.96	ns	1.04	ns	1.11	< 0.01
11807	Apoa2	0.97	ns	1.21	< 0.05	1.16	ns	1.02	ns
66113	Apoa5	0.94	ns	1.32	< 0.05	1.00	ns	0.92	ns
11814	Apoc3	0.98	ns	0.94	ns	0.98	ns	1.15	< 0.01
18830	PLTP	2.32	< 0.01	0.75	ns	0.64	ns	1.26	ns
Fatty acid transport
14081	Acsl1	0.97	ns	0.97	ns	0.82	ns	0.84	ns
74205	Acsl3	1.03	ns	1.01	ns	0.98	ns	0.94	ns
50790	Acsl4	1.14	< 0.05	0.98	ns	1.18	< 0.05	1.03	ns
433256	Acsl5	1.09	ns	1.02	ns	1.05	ns	1.12	ns
216739	Acsl6	0.97	ns	0.99	ns	1.01	ns	1.06	ns
12491	Cd36	1.61	< 0.01	1.06	ns	1.16	ns	1.42	< 0.05
13167	Dbi	0.86	ns	0.92	ns	1.39	< 0.01	1.08	ns
14080	Fabp1	1.09	ns	1.04	ns	0.97	ns	0.87	< 0.05
14079	Fabp2	1.05	ns	1.08	ns	0.95	ns	0.85	< 0.01
14077	Fabp3	1.36	< 0.05	1.15	< 0.05	1.83	< 0.01	0.90	ns
11770	Fabp4	1.00	ns	1.01	ns	1.26	ns	0.95	ns
16592	Fabp5	1.47	ns	0.88	ns	1.38	ns	0.99	ns
16204	Fabp6	0.94	< 0.01	1.02	ns	1.02	ns	1.04	ns
12140	Fabp7	1.07	ns	0.96	ns	0.97	ns	0.96	ns
16956	Lpl	0.85	< 0.01	1.02	ns	1.04	ns	0.95	ns
108078	Olr1	0.96	ns	1.01	ns	0.99	ns	0.95	ns
26457	Slc27a1	0.75	ns	0.97	ns	0.99	ns	1.03	ns
26458	Slc27a2	1.02	ns	0.97	ns	0.97	ns	1.15	ns
26569	Slc27a4	1.07	ns	1.01	ns	0.92	< 0.05	0.99	ns
26459	Slc27a5	0.99	ns	1.06	ns	1.00	ns	0.83	< 0.01
225579	Slc27a6	1.14	< 0.01	0.82	< 0.01	0.96	ns	1.11	< 0.05
Fatty acid oxidation
113868	Acaa1a	0.78	< 0.05	0.97	ns	1.46	< 0.05	1.15	< 0.05
11363	Acadl	0.91	ns	1.1	ns	1.09	ns	1.18	ns
11364	Acadm	0.7	ns	1.89	< 0.01	0.93	ns	1.08	ns
11430	Acox1	1.07	ns	1.74	ns	1.06	ns	0.67	ns
93732	Acox2	1.05	ns	1.76	< 0.01	0.97	ns	0.80	ns
80911	Acox3	0.99	ns	1.01	ns	0.99	ns	0.99	ns
12894	Cpt1a	0.98	ns	1.01	ns	0.99	ns	0.94	ns
12895	Cpt1b	1.17	ns	0.99	ns	1.20	< 0.05	1.07	ns
78070	Cpt1c	0.96	ns	1.01	ns	0.97	ns	0.98	< 0.05
12896	Cpt2	0.71	< 0.01	0.63	< 0.01	1.61	< 0.05	1.85	< 0.01
13117	Cyp4A10	1.00	ns	0.24	< 0.01	0.98	ns	5.07	< 0.01
13118	Cyp4a12b	1.03	ns	0.80	ns	0.94	ns	0.59	< 0.05
13119	Cyp4a14	0.90	< 0.01	0.06	< 0.01	1.06	ns	31.29	< 0.01
74147	Ehhadh	0.98	ns	0.69	< 0.05	0.89	ns	2.17	< 0.01
20280	Scp2	0.85	ns	0.95	ns	0.87	ns	0.91	ns
Ketogenesis
15360	Hmgcs2	0.92	ns	1.27	ns	1.72	< 0.01	1.14	< 0.05
Lipogenesis
56473	Fads2	1.05	ns	0.69	ns	0.93	ns	3.16	< 0.01
677317	Mod1	0.58	< 0.01	0.75	ns	2.26	< 0.01	2.81	< 0.01
20249	Scd1	0.76	< 0.01	0.81	< 0.05	1.45	< 0.01	1.33	< 0.01
20250	Scd2	1.26	ns	1.46	< 0.01	2.03	< 0.01	1.16	ns
30049	Scd3	1.03	ns	0.98	ns	0.98	ns	1.02	ns
329065	Scd4	1.01	ns	1.06	ns	0.98	ns	1.00	ns
Cholesterol metabolism
104086	Cyp27a1	1.04	ns	1.00	ns	0.78	< 0.01	0.70	< 0.01
13122	Cyp7a1	0.96	ns	2.31	ns	1.00	ns	0.49	ns
13124	Cyp8b1	1.00	ns	1.37	< 0.05	0.99	ns	1.04	ns
22259	Nr1h3	0.96	ns	0.92	ns	0.76	< 0.05	1.11	ns
Gluconeogenesis
11832	Aqp7	0.83	ns	1.04	ns	1.12	ns	1.03	ns
14626	Gk2	1.02	ns	1.01	ns	1.05	ns	1.03	ns
14933	Gyk	1.01	ns	0.96	ns	1.00	ns	1.00	ns
18534	Pck1	0.65	ns	0.54	ns	0.87	ns	1.15	ns
74551	Pck2	0.99	ns	1.17	< 0.01	0.97	ns	0.86	< 0.05
Adipocyte differentiation
11450	Adipoq	0.71	< 0.05	0.97	ns	0.49	< 0.01	1.01	ns
57875	Angptl4	1.41	ns	0.84	ns	0.71	< 0.01	1.04	ns
83995	Mmp1a	1.02	ns	0.99	ns	0.99	ns	1.00	ns
83996	Mmp1b	1.06	ns	0.97	ns	0.97	ns	1.04	ns
103968	Plin	0.70	ns	0.98	ns	0.68	ns	1.03	ns
20411	Sorbs1	0.95	ns	0.94	ns	1.04	ns	1.06	ns
Adaptive thermogenesis
22227	Ucp1	2.65	< 0.01	1.03	ns	2.87	< 0.01	0.98	ns
Cell survival
16202	Ilk	1.27	ns	0.83	ns	0.77	ns	1.14	< 0.05
18607	Pdpk1	0.62	< 0.01	1.38	< 0.01	1.61	< 0.05	0.83	< 0.05
Ubiquitination
22190	Ubc	0.87	ns	1.25	ns	0.83	ns	0.76	ns

Changes in expression are expressed as fold changes in BFMI860 in comparison to B6 mice on SMD, and of HFD-fed BFMI860 mice compared to SMD-fed mice, respectively. SMD- standard maintenance diet, HFD- high-fat diet, ns-not significant

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