Effect of Ganoderma lucidum spores intervention on glucose and lipid metabolism gene expression profiles in type 2 diabetic rats

Table 4 The primer of genes

Gene	Accession number	Forward primes(5′–3′)	Reverse primes(5′–3′)	Amplicon product (bp)	Annealing temperature (°C)
18S rRNA	NR_046237	AAACGGCTACCACATCCAAG	TTGCCCTCCAATGGATCCT	159	60
ACC	NM_022193	CAACCACTACGGCATGACTCA	CGCAGAAGCAGCCCATTACTT	155	60
FAS	NM_017332	TGCTCCCAGCTGCAG	GCCCGGTAGCTCTGGGTGTA	107	60
Acox1	NM_017340	CAAGGAGAGTGCTACGGGTTA	TTCAGGTAGCCGTTATCCAT	137	58
SREBP-1	XM_213329	GCAAGGCCATCGACTACATC	TTTCATGCCCTCCATAGACAC	161	60
Insig1	NM_022392	TTGTCGGCTTATTGTATCCCT	GCACATTATTGGCGAAATCT	147	55
Insig2	NM_178091	GGCGGAAGGAGAGACGGAGTC	AAGCCAGGAACACGCCAATGA	128	60
Acly	NM_016987	GCAGACCAGAAGGGCGTGAC	CACACTGCCTGGGCGATACAG	135	64
Fads1	NM_053445	GTTTGTGTGGGTGACGCAGAT	TTGAAGGCTGACTGGTGAACG	117	60
Gpam	NM_017274	CCTGTGGGCATCTCGTATGAT	TTCCGCAGCATTCTGATAAC	122	60
Dgat1	NM_053437	CAGATGGGGCTGCTGCTACAT	GGCGGCACCACAGATTGACAT	175	60
GS2	NM_013089	GACACTGAGCAGGGCTTTTCC	GAGGAGGGCCTGGGATACTT	90	60
GYG1	NM_031043.2	TCGCCAGCCCACAGGTT	CACCACTGTCCAAGACATCTACCA	90	60
PEPCK	NM_198780	GAAAGTTGAATGTGTGGGTGAT	TTCTGGGTTGATGGCCCTTA	79	57
G6PC1	NM_013098	GTATGGATTCCGGTGCTT	AATGCCTGACAAGACTCCA	132	55

ISSN: 1476-511X